Microfluidic system for monitoring of cardiac (H9C2) cell proliferation.

The paper presents the application of electrical impedance spectroscopy (EIS) analysis for investigation of cardiac cell (H9C2 – rat cardiomyoblast) proliferation after verapamil hydrochloride exposure. For this purpose, two different PDMS/glass microsystems with circular microchamber and longitudinal microchannel integrated with Pt/Al electrodes were used. The microchambers were fabricated in PDMS using photolithography and replica moulding techniques. Pt/Al electrodes were fabricated on a 4-inch glass substrate using Physical Vapor Deposition (PVD). Solution of verapamil hydrochloride was continuously introduced into the microsystems with H9C2 cell culture (a flow rate of 1 μl/min) for 72 h. The impedance spectra were recorded from 100 Hz to 1 MHz. We confirmed that impedance spectroscopy can be used for non-invasive, label-free and real-time analysis of cardiac cells proliferation based on cells dielectric properties and biological structure.


Published in:
Proc. SPIE, 10247, 102470E-102470E
Presented at:
Bio-MEMS and Medical Microdevices III, Barcelona, Spain, May 08, 2017
Year:
2017
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2017-07-10, last modified 2018-09-13

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