Multiple embryo time-lapse imaging of zebrafish development.

Understanding the dynamics of developmental and cellular processes requires documentation of their changes with appropriate temporal and spatial resolution. Furthermore, simultaneous recording from a population of embryos under identical conditions allows statistical estimates of precision and variability to be made. This chapter describes a protocol for time-lapse microscopy of multiple embryos in parallel developing under tightly controlled conditions. This method is currently best suited to follow tissue-scale morphogenetic movements with temporal resolution in the minute range, for hours or even days. Applications of the method include the comparison of the dynamics of a process of interest between groups of wild-type embryos and their mutant siblings or between embryos treated with different chemical compounds. Temperature control allows for the investigation of the temperature dependence of a process of interest.


Published in:
Methods in molecular biology (Clifton, N.J.), 546, 243-254
Year:
2009
ISSN:
10643745
Keywords:
Laboratories:




 Record created 2017-05-30, last modified 2018-01-28

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