000228086 001__ 228086
000228086 005__ 20180913064325.0
000228086 013__ $$aWO2016131833$$bWO$$cA1$$d20160825
000228086 02470 $$2EPO Family ID$$a52477639
000228086 02470 $$2TTO$$a6.1470
000228086 037__ $$aPATENT
000228086 245__ $$aSensors, methods and kits for detecting nicotinamide adenine dinucleotides
000228086 260__ $$c2016
000228086 269__ $$a2016
000228086 336__ $$aPatents
000228086 520__ $$aThe invention relates to the in vitro and in cellulo detection of the cofactors nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Provided is a sensor molecule for fluorescence or luminescence-based detection of a nicotinamide adenine dinucleotide analyte, in particular for detecting the concentrations of NAD+, NADP' and/or the ratios of the concentrations of NAD '/NAD H and NADP '/NADPH, the sensor comprising (i) a binding protein (BP) for the nicotinamide adenine dinucleotide analyte, the BP being derived from sepiapterin reductase (SPR; EC 1.1.1.153) (ii) a SPR ligand (SPR-L) capable of intramolecular binding to said BP in the presence of the oxidized form of said analyte; and (iii) at least one fluorophore.
000228086 700__ $$0246115$$aSallin, Olivier$$g191792
000228086 700__ $$0240935$$aReymond, Luc$$g155775
000228086 700__ $$aJohnsson, Kai Peter
000228086 909C0 $$0252085$$pTTO$$xU10021
000228086 909C0 $$0252027$$pLIP$$xU10102
000228086 909CO $$ooai:infoscience.tind.io:228086$$pSB
000228086 917Z8 $$x105423
000228086 937__ $$aEPFL-PATENT-228086
000228086 973__ $$aEPFL
000228086 980__ $$aPATENT