The telomeric DNA damage response occurs in the absence of chromatin decompaction

Telomeres are specialized nucleoprotein structures that protect chromosome ends from DNA damage response (DDR) and DNA rearrangements. The telomeric shelterin protein TRF2 suppresses the DDR, and this function has been attributed to its abilities to trigger t-loop formation or prevent massive decompaction and loss of density of telomeric chromatin. Here, we applied stochastic optical reconstruction microscopy (STORM) to measure the sizes and shapes of functional human telomeres of different lengths and dysfunctional telomeres that elicit a DDR. Telomeres have an ovoid appearance with considerable plasticity in shape. Examination of many telomeres demonstrated that depletion of TRF2, TRF1, or both affected the sizes of only a small subset of telomeres. Costaining of telomeres with DDR markers further revealed that the majority of DDR signaling telomeres retained a normal size. Thus, DDR signaling at telomeres does not require decompaction. We propose that telomeres are monitored by the DDR machinery in the absence of telomere expansion and that the DDR is triggered by changes at the molecular level in structure and protein composition.


Published in:
Genes and Development, 31, 6, 567-577
Year:
2017
Publisher:
Cold Spring Harbor, Cold Spring Harbor Laboratory
ISSN:
0890-9369
1549-5477
Keywords:
Note:
This article is licensed under a Creative Commons Attribution 4.0 International License
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Note: The status of this file is: Anyone


 Record created 2017-04-20, last modified 2020-07-29

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