000226996 001__ 226996
000226996 005__ 20181203024622.0
000226996 0247_ $$2doi$$a10.5435/Jaaos-D-16-00630
000226996 022__ $$a1067-151X
000226996 02470 $$2ISI$$a000393541800003
000226996 037__ $$aARTICLE
000226996 245__ $$aPreclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach to Detect an Orthopaedic Implant Infection
000226996 260__ $$bLippincott Williams & Wilkins$$c2017$$aPhiladelphia
000226996 269__ $$a2017
000226996 300__ $$a6
000226996 336__ $$aJournal Articles
000226996 520__ $$aIntroduction: Diagnosing-prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for diagnosing PJI safely and noninvasively. Materials: In an established preclinical mouse model of bioluminescent Staphylococcus aureus PJI, fluorescent indocyanine green (ICG) was conjugated to beta-cyclodextrin (CDX-ICG) or teicoplanin (Teic-ICG) and injected intravenously for 1 week postoperatively. Daily fluorescent imaging and PAI were used to localize and quantify tracer signals. Results were analyzed using 2-way analysis of variance. Results: Fluorescence clearly localized to the site of infection and was significantly higher with Teic-ICG compared with CDX-ICG (P = 0.046) and ICG alone (P = 0.0087). With PAI, the photoacoustic signal per volumetric analysis was substantially higher and better visualized with Teic-ICG compared with CDX-ICG and ICG alone, and colocalized well with bioluminescence and fluorescence imaging. Conclusion: Photoacoustic imaging successfully localized PJI in this proof-of concept study and demonstrates potential for clinical translation in orthopaedics.
000226996 700__ $$uWashington Univ, St Louis, MO 63130 USA$$aWang, Yu
000226996 700__ $$uJohns Hopkins Univ Hosp, Div Infect Dis, Baltimore, MD 21287 USA$$aThompson, John M.
000226996 700__ $$uUniv Calif Los Angeles, Los Angeles, CA USA$$aAshbaugh, Alyssa G.
000226996 700__ $$uEcole Polytech Fed Lausanne, Lausanne, Switzerland$$aKhodakivskyi, Pavlo
000226996 700__ $$uIntrace Med SA, Lausanne, Switzerland$$aBudin, Ghyslain
000226996 700__ $$uIntrace Med SA, Lausanne, Switzerland$$aSinisi, Riccardo
000226996 700__ $$uFUJIFILM VisualSon, Toronto, ON, Canada$$aHeinmiller, Andrew
000226996 700__ $$aVan Oosten, Marleen
000226996 700__ $$aVan Dijl, Jan Maarten
000226996 700__ $$aVan Dam, Gooitzen M.
000226996 700__ $$uPerkinsElmer, Waltham, MA USA$$aFrancis, Kevin P.
000226996 700__ $$uRonald Reagan Univ Calif, Los Angeles Med Ctr, Santa Monica, CA USA$$aBernthal, Nicholas M.
000226996 700__ $$aDubikovskaya, Elena A.
000226996 700__ $$uJohns Hopkins Univ Hosp, Div Infect Dis, Baltimore, MD 21287 USA$$aMiller, Lloyd S.
000226996 773__ $$j25$$tJournal Of The American Academy Of Orthopaedic Surgeons$$qS7-S12
000226996 909C0 $$xU10095$$0252439$$pISIC
000226996 909CO $$pSB$$particle$$ooai:infoscience.tind.io:226996
000226996 917Z8 $$x231591
000226996 937__ $$aEPFL-ARTICLE-226996
000226996 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000226996 980__ $$aARTICLE