000226859 001__ 226859
000226859 005__ 20180317094452.0
000226859 0247_ $$2doi$$a10.1371/journal.pone.0172943
000226859 022__ $$a1932-6203
000226859 02470 $$2ISI$$a000396011300049
000226859 037__ $$aARTICLE
000226859 245__ $$aSingle particle maximum likelihood reconstruction from superresolution microscopy images
000226859 260__ $$aSan Francisco$$bPublic Library Science$$c2017
000226859 269__ $$a2017
000226859 300__ $$a18
000226859 336__ $$aJournal Articles
000226859 520__ $$aPoint localization superresolution microscopy enables fluorescently tagged molecules to be imaged beyond the optical diffraction limit, reaching single molecule localization precisions down to a few nanometers. For small objects whose sizes are few times this precision, localization uncertainty prevents the straightforward extraction of a structural model from the reconstructed images. We demonstrate in the present work that this limitation can be overcome at the single particle level, requiring no particle averaging, by using a maximum likelihood reconstruction (MLR) method perfectly suited to the stochastic nature of such superresolution imaging. We validate this method by extracting structural information from both simulated and experimental PALM data of immature virus-like particles of the Human Immunodeficiency Virus (HIV-1). MLR allows us to measure the radii of individual viruses with precision of a few nanometers and confirms the incomplete closure of the viral protein lattice. The quantitative results of our analysis are consistent with previous cryoelectron microscopy characterizations. Our study establishes the framework for a method that can be broadly applied to PALM data to determine the structural parameters for an existing structural model, and is particularly well suited to heterogeneous features due to its single particle implementation.
000226859 700__ $$aVerdier, Timothee$$uUniv Claude Bernard, Ens Lyon, CNRS, Univ Lyon,Lab Phys, F-69342 Lyon, France
000226859 700__ $$0245032$$aGunzenhauser, Julia$$g166106
000226859 700__ $$0244708$$aManley, Suliana$$g189937
000226859 700__ $$aCastelnovo, Martin$$uUniv Claude Bernard, Ens Lyon, CNRS, Univ Lyon,Lab Phys, F-69342 Lyon, France
000226859 773__ $$j12$$k3$$qe0172943$$tPlos One
000226859 909CO $$ooai:infoscience.tind.io:226859$$particle$$pSB
000226859 909C0 $$0252362$$pLEB$$xU12031
000226859 937__ $$aEPFL-ARTICLE-226859
000226859 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000226859 980__ $$aARTICLE