000226696 001__ 226696
000226696 005__ 20190503101423.0
000226696 022__ $$a2045-2322
000226696 02470 $$2ISI$$a000396331500001
000226696 0247_ $$2doi$$a10.1038/srep44409
000226696 037__ $$aARTICLE
000226696 245__ $$aAptamer-based Field-Effect Biosensor for Tenofovir Detection
000226696 260__ $$c2017-03-15
000226696 269__ $$a2017-03-15
000226696 300__ $$a10
000226696 336__ $$aJournal Articles
000226696 520__ $$aDuring medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target. Surface plasmon resonance, performed under different buffer conditions, shows optimum specific and little non-specific binding in phosphate buffered saline. The dose-response behavior of the field-effect biosensor presents a linear range between 1 nM and 100 nM of tenofovir and a limit of detection of 1.2 nM. Two non-specific drugs and one non-specific aptamer, tested as stringent control candidates, caused negligible responses. The applications were successfully extended to the detection of the drug in human serum. As demonstrated by impedance measurements, the aptamer-based sensors can be used for real-time drug monitoring.
000226696 536__ $$aH2020$$cERC Cybercare 669354
000226696 536__ $$aEU funding$$cMarie-Curie Initial Training Network PROSENSE 317420
000226696 700__ $$0247699$$aAliakbari, Nima$$g224484
000226696 700__ $$aJolly, Pawan
000226696 700__ $$aBhalla, Nikhil
000226696 700__ $$aMiodek, Anna
000226696 700__ $$0240269$$aDe Micheli, Giovanni$$g167918
000226696 700__ $$aEstrela, Pedro
000226696 700__ $$0242413$$aCarrara, Sandro$$g182237
000226696 773__ $$j7$$k44409$$tScientific Reports
000226696 8564_ $$s906358$$uhttps://infoscience.epfl.ch/record/226696/files/srep44409.pdf$$yn/a$$zn/a
000226696 8560_ $$fchantal.demont@epfl.ch
000226696 909C0 $$0252283$$pLSI1$$xU11140
000226696 909CO $$ooai:infoscience.tind.io:226696$$pSTI$$pIC$$particle$$qGLOBAL_SET
000226696 917Z8 $$x112915
000226696 937__ $$aEPFL-ARTICLE-226696
000226696 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000226696 980__ $$aARTICLE