4D Cryo-Electron Microscopy of Proteins

Cryo-electron microscopy is a form of transmission electron microscopy that has been used to determine the 3D structure of biological specimens in the hydrated state and with high resolution. We report the development of 4D cryo-electron microscopy by integrating the fourth dimension, time, into this powerful technique. From time-resolved diffraction of amyloid fibrils in a thin layer of vitrified water at cryogenic temperatures, we were able to detect picometer movements of protein molecules on a nanosecond time scale. Potential future applications of 4D cryo-electron microscopy are numerous, and some are discussed here. © 2013 American Chemical Society.


Published in:
Journal of the American Chemical Society, 135, 51, 19123-19126
Year:
2013
Laboratories:




 Record created 2017-02-13, last modified 2018-09-13


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