000225207 001__ 225207
000225207 005__ 20180913064149.0
000225207 0247_ $$2doi$$a10.1002/btpr.2319
000225207 022__ $$a8756-7938
000225207 02470 $$2ISI$$a000387787600025
000225207 037__ $$aARTICLE
000225207 245__ $$aMultigene expression in stable CHO cell pools generated with the piggyBac transposon system
000225207 260__ $$aHoboken$$bAmerican Chemical Society$$c2016
000225207 269__ $$a2016
000225207 300__ $$a10
000225207 336__ $$aJournal Articles
000225207 520__ $$aHeterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of three model proteinsenhanced green fluorescent protein (EGFP), secreted alkaline phosphatase (SEAP), and a monoclonal IgG1 antibody. Each transgene was present on a separate PB donor plasmid with either the same or a different selection gene. In both cases, we obtained PB-derived cell pools with higher recombinant protein yields than from cell pools generated by conventional gene delivery. In PB-derived cell pools generated using a single selection agent, both protein production and the number of integrated copies of each transgene declined as the number of transfected transgenes increased. However, the total number of integrated transgenes was similar regardless of the number of different transgenes transfected. For PB-derived cell pools generated by selection of each transgene with a different selection agent, the total number of integrated transgenes increased with the number of transfected transgenes. The results suggest that the generation of cell pools producing multiple recombinant proteins is feasible and that the method is more efficient when each individual transgene is selected with a different marker. (c) 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1308-1317, 2016
000225207 6531_ $$apiggyBac
000225207 6531_ $$aCHO
000225207 6531_ $$arecombinant protein
000225207 6531_ $$astable gene expression
000225207 6531_ $$acell pools
000225207 700__ $$aBalasubramanian, Sowmya$$uEcole Polytech Fed Lausanne, Lab Cellular Biotechnol LBTC, CH-1015 Lausanne, Switzerland
000225207 700__ $$0240398$$aWurm, Florian M.$$g107554$$uEcole Polytech Fed Lausanne, Lab Cellular Biotechnol LBTC, CH-1015 Lausanne, Switzerland
000225207 700__ $$0240882$$aHacker, David L.$$g152132$$uEcole Polytech Fed Lausanne, Lab Cellular Biotechnol LBTC, CH-1015 Lausanne, Switzerland
000225207 773__ $$j32$$k5$$q1308-1317$$tBiotechnology Progress
000225207 909C0 $$0252009$$pLBTC$$xU10180
000225207 909CO $$ooai:infoscience.tind.io:225207$$particle
000225207 917Z8 $$x117359
000225207 937__ $$aEPFL-ARTICLE-225207
000225207 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000225207 980__ $$aARTICLE