000225022 001__ 225022
000225022 005__ 20181203024531.0
000225022 0247_ $$2doi$$a10.1371/journal.pone.0167059
000225022 022__ $$a1932-6203
000225022 02470 $$2ISI$$a000389472400077
000225022 037__ $$aARTICLE
000225022 245__ $$aMycobacterium ulcerans Mouse Model Refinement for Pre-Clinical Profiling of Vaccine Candidates
000225022 260__ $$bPublic Library of Science$$c2016$$aSan Francisco
000225022 269__ $$a2016
000225022 300__ $$a15
000225022 336__ $$aJournal Articles
000225022 520__ $$aBuruli Ulcer is a neglected tropical disease leading to extensive disabilities and morbidity in West Africa. In this paper we sought to characterize various strains of Mycobacterium ulcerans (M. ulcerans) with different origins and laboratory passage records while refining a mouse model for Buruli ulcer. We described, compared and followed the kinetics of the histo-pathological outcome of infection of a collection of strains at various anatomical sites of infection in order to find a suitable model for further immunization studies. Moreover we compared the outcome of infection in C57Bl/6 and Balbc/J mice. Specifically we described thoroughly one M. ulcerans strain characterized by slow growth rate and limited tissue necrosis, which presents close ressemblance with the infection kinetics in humans. This strain caused macrophages as well as T and B cells infiltration, correlating with mycobacterial proliferation at the site of infection as well as in the draining lymph nodes, making it a suitable strain to screen vaccine candidates efficacy.
000225022 700__ $$uSwiss Trop & Publ Hlth Inst, Basel, Switzerland$$aBenard, Angele
000225022 700__ $$0244366$$g179660$$uEcole Polytech Fed Lausanne, Global Hlth Inst, Lausanne, Switzerland$$aSala, Claudia
000225022 700__ $$aPluschke, Gerd$$uSwiss Trop & Publ Hlth Inst, Basel, Switzerland
000225022 773__ $$j11$$tPlos One$$k11
000225022 909C0 $$xU11742$$0252302$$pUPCOL
000225022 909CO $$pSV$$particle$$ooai:infoscience.tind.io:225022
000225022 917Z8 $$x282550
000225022 937__ $$aEPFL-ARTICLE-225022
000225022 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000225022 980__ $$aARTICLE