Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor

The mouse serotonin 5-HT3A receptor is a homo-pentameric ligand-gated ion channel (pLGIC) mediating fast excitatory neurotransmission in the central nervous system. The molecular mechanism of ion permeation of 5-HT3A receptors triggered by the neurotransmitter serotonin is not yet fully understood. The recent X-ray structure of the mouse serotonin 5-HT3A receptor in complex with a stabilizing nanobody revealed for the first time the entire structure of a mammalian pLGIC in detergent. Structural information of the receptor in a lipid bilayer however is still limited primarily due to the lack of 2D crystals of the receptor in a lipid bilayer. Here we present our results on the formation and improvement of diffracting 2D crystals of the mouse 5-HT3A by limited proteolysis and addition of conformational nanobodies. (C) 2016 Elsevier Ltd. All rights reserved.


Published in:
Micron, 92, 19-24
Year:
2017
Publisher:
Oxford, Pergamon-Elsevier Science Ltd
ISSN:
0968-4328
Keywords:
Laboratories:




 Record created 2017-01-18, last modified 2018-09-13


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