000223191 001__ 223191
000223191 005__ 20190317000559.0
000223191 0247_ $$2doi$$a10.1038/ncomms13121
000223191 022__ $$a2041-1723
000223191 02470 $$2ISI$$a000385729700001
000223191 037__ $$aARTICLE
000223191 245__ $$aA centrifugation-based physicochemical characterization method for the interaction between proteins and nanoparticles
000223191 269__ $$a2016
000223191 260__ $$bNature Publishing Group$$c2016$$aLondon
000223191 300__ $$a8
000223191 336__ $$aJournal Articles
000223191 520__ $$aNanomedicine requires in-depth knowledge of nanoparticle-protein interactions. These interactions are studied with methods limited to large or fluorescently labelled nanoparticles as they rely on scattering or fluorescence-correlation signals. Here, we have developed a method based on analytical ultracentrifugation (AUC) as an absorbance-based, label-free tool to determine dissociation constants (K-D), stoichiometry (N-max), and Hill coefficient (n), for the association of bovine serum albumin (BSA) with gold nanoparticles. Absorption at 520 nm in AUC renders the measurements insensitive to unbound and aggregated proteins. Measurements remain accurate and do not become more challenging for small (sub-10 nm) nanoparticles. In AUC, frictional ratio analysis allows for the qualitative assessment of the shape of the analyte. Data suggests that small-nanoparticles/protein complexes significantly deviate from a spherical shape even at maximum coverage. We believe that this method could become one of the established approaches for the characterization of the interaction of (small) nanoparticles with proteins.
000223191 700__ $$0247324$$g233468$$uEcole Polytech Fed Lausanne, Inst Mat, CH-1015 Lausanne, Switzerland$$aBekdemir, Ahmet
000223191 700__ $$uEcole Polytech Fed Lausanne, Inst Mat, CH-1015 Lausanne, Switzerland$$aStellacci, Francesco$$g196998$$0244724
000223191 773__ $$j7$$tNature Communications$$q13121
000223191 8564_ $$uhttps://infoscience.epfl.ch/record/223191/files/ncomms13121.pdf$$zn/a$$s558790$$yn/a
000223191 909C0 $$xU12173$$0252323$$pSUNMIL
000223191 909CO $$ooai:infoscience.tind.io:223191$$qGLOBAL_SET$$pSTI$$particle
000223191 917Z8 $$x196998
000223191 937__ $$aEPFL-ARTICLE-223191
000223191 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000223191 980__ $$aARTICLE