Biomimetic surface patterning for long-term transmembrane access

Here we present a planar patch clamp chip based on biomimetic cell membrane fusion. This architecture uses nanometer length-scale surface patterning to replicate the structure and function of membrane proteins, creating a gigaohm seal between the cell and a planar electrode array. The seal is generated passively during cell spreading, without the application of a vacuum to the cell surface. This interface can enable cell-attached and whole-cell recordings that are stable to 72 hours, and generates no visible damage to the cell. The electrodes can be very small (<5 μm) and closely packed, offering a high density platform for cellular measurement.


Published in:
Scientific Reports, 6, 32485
Year:
2016
Publisher:
London, Nature Publishing Group
ISSN:
2045-2322
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2016-10-07, last modified 2018-03-17

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