Significant research in tissue engineering has been devoted to the replication of cartilage by developing suitable scaffolds. The approach of Ren et al was to develop an injectable Hyaluronic acid-tyramine hydrogel. HA-tyr hydrogels can be crosslinked by adding hydrogen-peroxide (H2O2) and the enzyme horse-radish peroxidase (HRP). However, the reaction is almost instantaneous and temporal control is difficult. By separating HRP from HA-Tyr and H2O2, the mixture is liquid. Hence, the main goal of this project was to develop a thermoresponsive system for HRP delivery aiming to trigger and control HA-Tyr crosslinking reaction.