Development of thermoresponsive liposomes as a delivery system of HRP
Significant research in tissue engineering has been devoted to the replication of cartilage by developing suitable scaffolds. The approach of Ren et al was to develop an injectable Hyaluronic acid-tyramine hydrogel. HA-tyr hydrogels can be crosslinked by adding hydrogen-peroxide (H2O2) and the enzyme horse-radish peroxidase (HRP). However, the reaction is almost instantaneous and temporal control is difficult. By separating HRP from HA-Tyr and H2O2, the mixture is liquid. Hence, the main goal of this project was to develop a thermoresponsive system for HRP delivery aiming to trigger and control HA-Tyr crosslinking reaction.