Fluorogenic Probes for Multicolor Imaging in Living Cells

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 nm and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes and SNAP-tag are fluorogenic, cell-permeable and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.


Published in:
Journal of the American Chemical Society
Year:
2016
Publisher:
Washington, American Chemical Society
ISSN:
1520-5126
Laboratories:




 Record created 2016-07-27, last modified 2018-03-17


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