000220016 001__ 220016
000220016 005__ 20190317000506.0
000220016 0247_ $$2doi$$a10.5075/epfl-thesis-7121
000220016 02470 $$2urn$$aurn:nbn:ch:bel-epfl-thesis7121-7
000220016 02471 $$2nebis$$a10684072
000220016 037__ $$aTHESIS
000220016 041__ $$aeng
000220016 088__ $$a7121
000220016 245__ $$aThin films for Healthcare Acquired Infections (HAIs) prevention$$bmaterials preparation, testing and characterization
000220016 260__ $$aLausanne$$bEPFL$$c2016
000220016 269__ $$a2016
000220016 300__ $$a168
000220016 336__ $$aTheses
000220016 502__ $$aDr Horst Pick (président) ; Prof. César Pulgarin (directeur de thèse) ; Prof. Paul Bowen, Prof. Suresh Pillai, Prof. Dionysios Dionysiou (rapporteurs)
000220016 520__ $$aThis thesis addresses the preparation of antibacterial surfaces for hospital facilities taking biomedical thin polymer films, textiles and catheter as probes. Magnetron sputtering is used to apply the coatings leading to fast bacterial inactivation in the dark and under low intensity light on the selected substrates. These coating are designed and prepared to achieve fast bacterial inactivation to avoid biofilm formation on textiles and polymer films and later on the catheter surface. The infection due to catheters is one of the major problem leading to catheter associated infections (CAIs). The design, preparation, testing and the characterization of the surface properties of uniform and adhesive TiO2,TiO2/Cu, Ag, Cu-films presenting fast bacterial inactivation kinetics on textiles and catheters by up to date HIPIMS and DCMS/DCPMS has been addressed very sparsely until now mainly on 3D objects. No evidence for Ag-Cu coating applied by sputtering covering uniformly the entire catheter surface has been reported up to this date. A modification of the sputtering unit was carried out to coat 3D objects and is one of the main novelties reported in this thesis. The antimicrobial activity was tested on antibiotic resistant bacteria Methycillin resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli) alone or jointly representing an important focus of infection by themselves or in the form of pathogenic biofilms in hospital facilities. The bacterial inactivation kinetics was investigated in details under low intensity light and in the dark. The active part of the catheter remains under the patient skin (in the dark). Ag/Cu-has been selected since fast bacterial inactivation proceeds in with a quasi-instantaneous kinetics inducing a cytotoxicity below the limit authorized by the sanitary regulations for mammalian cells. These coatings are also well tolerated by osteoblasts. The sputtered films leading to fast bacterial inactivation and showing low cytotoxicity consisted mainly of TiO2, Cu-TiO2, and Ag/Cu films on 2D surfaces and on catheters with 3D-geometry. This is the first report for materials of this kind, their evaluation and surface properties.
000220016 6531_ $$aBiomedical textile
000220016 6531_ $$aCatheters
000220016 6531_ $$aHospital Acquired Infections (HAIs)
000220016 6531_ $$aAg/Cu-sputtering
000220016 6531_ $$a3D-geometry
000220016 6531_ $$aBacterial inactivation in the dark
000220016 6531_ $$aOligodynamic effect.
000220016 700__ $$0247403$$aRtimi, Sami$$g216848
000220016 720_2 $$0240005$$aPulgarin, César$$edir.$$g106159
000220016 8564_ $$s7322564$$uhttps://infoscience.epfl.ch/record/220016/files/EPFL_TH7121.pdf$$yn/a$$zn/a
000220016 909CO $$ooai:infoscience.tind.io:220016$$pthesis$$pthesis-bn2018$$pDOI$$qDOI2$$qGLOBAL_SET
000220016 917Z8 $$x108898
000220016 917Z8 $$x108898
000220016 917Z8 $$x108898
000220016 917Z8 $$x108898
000220016 918__ $$aSB$$cISIC$$dEDCH
000220016 919__ $$aGR-PUL
000220016 920__ $$a2016-7-29$$b2016
000220016 970__ $$a7121/THESES
000220016 973__ $$aEPFL$$sPUBLISHED
000220016 980__ $$aTHESIS