000219704 001__ 219704
000219704 005__ 20190416220331.0
000219704 0247_ $$2doi$$a10.1016/j.celrep.2016.03.088
000219704 022__ $$a2211-1247
000219704 02470 $$2ISI$$a000376164600001
000219704 037__ $$aARTICLE
000219704 245__ $$aWnt Ligands Secreted by Subepithelial Mesenchymal Cells Are Essential for the Survival of Intestinal Stem Cells and Gut Homeostasis
000219704 269__ $$a2016
000219704 260__ $$aCambridge$$bElsevier$$c2016
000219704 300__ $$a8
000219704 336__ $$aJournal Articles
000219704 520__ $$aTargeting of Wnt signaling represents a promising anti-cancer therapy. However, the consequences of systemically attenuating the Wnt pathway in an adult organism are unknown. Here, we globally prevent Wnt secretion by genetically ablating Wntless. We find that preventing Wnt signaling in the entire body causes mortality due to impaired intestinal homeostasis. This is caused by the loss of intestinal stem cells. Reconstitution of Wnt/beta-catenin signaling via delivery of external Wnt ligands prolongs the survival of intestinal stem cells and reveals the essential role of extra-epithelial Wnt ligands for the renewal of the intestinal epithelium. Wnt2b is a key extra-epithelial Wnt ligand capable of promoting Wnt/beta-catenin signaling and intestinal homeostasis. Wnt2b is secreted by subepithelial mesenchymal cells that co-express either Gli1 or Acta2. Subepithelial mesenchymal cells expressing high levels of Wnt2b are predominantly Gli1 positive.
000219704 700__ $$aValenta, Tomas$$uUniv Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
000219704 700__ $$aDegirmenci, Bahar$$uUniv Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
000219704 700__ $$aMoor, Andreas E.$$uWeizmann Inst Sci, Dept Mol Cell Biol, Wolfson Bldg 623, IL-76100 Rehovot, Israel
000219704 700__ $$aHerr, Patrick$$uUniv Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
000219704 700__ $$aZimmerli, Dario$$uUniv Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
000219704 700__ $$aMoor, Matthias B.$$uUniv Lausanne, Dept Pharmacol & Toxicol, CH-1005 Lausanne, Switzerland
000219704 700__ $$aHausmann, George$$uUniv Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
000219704 700__ $$aCantu, Claudio$$uUniv Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
000219704 700__ $$0240585$$aAguet, Michel$$g168669$$uEcole Polytech Fed Lausanne, Sch Life Sci, Swiss Inst Expt Canc Res ISREC, CH-1015 Lausanne, Switzerland
000219704 700__ $$aBasler, Konrad$$uUniv Zurich, Inst Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
000219704 773__ $$j15$$k5$$q911-918$$tCell Reports
000219704 8564_ $$s3721382$$uhttps://infoscience.epfl.ch/record/219704/files/1-s2.0-S2211124716303941-main.pdf$$yPublisher's version$$zPublisher's version
000219704 909C0 $$0252150$$pUPAGU$$xU11156
000219704 909CO $$ooai:infoscience.tind.io:219704$$pSV$$particle$$qGLOBAL_SET
000219704 917Z8 $$x168669
000219704 917Z8 $$x182396
000219704 937__ $$aEPFL-ARTICLE-219704
000219704 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000219704 980__ $$aARTICLE