Nonstatistical UV Fragmentation of Gas-Phase Peptides Reveals Conformers and Their Structural Features

Solving the 3D structure of a biomolecule requires recognition of its conformers and measurements of their individual structural identities, which can be compared with calculations. We employ the phenomenon of nonstatistical photofragmentation, detected by a combination of UV cold ion spectroscopy and high-resolution mass spectrometry, to identify the main conformers of gas-phase peptides and to recover individual UV absorption and mass spectra of all of these conformers in a single laser scan. We first validate this approach with a benchmark dipeptide, Tyr-Ala, and then apply it to a decapeptide, gramicidin S. The revealed characteristic structural difference between the conformers of the latter identifies some of the previously calculated structures of gramicidin S as the most likely geometries of its remaining unsolved conformer.


Published in:
Journal of physical chemistry letters, 7, 1067-1071
Year:
2016
Publisher:
Washington, Amer Chemical Soc
ISSN:
1948-7185
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2016-07-13, last modified 2018-01-28

External link:
Download fulltext
n/a
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)