000219128 001__ 219128
000219128 005__ 20190317000458.0
000219128 0247_ $$2doi$$a10.1021/acs.analchem.6b01142
000219128 022__ $$a1520-6882
000219128 02470 $$2ISI$$a000378470200017
000219128 037__ $$aARTICLE
000219128 245__ $$aMass Barcode Signal Amplification for Multiplex Allergy Diagnosis by MALDI-MS
000219128 269__ $$a2016
000219128 260__ $$bAmerican Chemical Society$$c2016$$aWashington
000219128 300__ $$a6
000219128 336__ $$aJournal Articles
000219128 520__ $$aA highly sensitive method based on mass-barcoded gold nanoparticles (AuNPs) and immunomagnetic separation has been developed for multiplex allergy diagnosis by MALDI mass spectrometry in a component-resolved manner. Different analytical probes were prepared by coating AuNPs with individual allergenic proteins and mass barcode, represented by polyethylene glycol molecules of various chain lengths. Magnetic beads (MBs) functionalized with antihuman IgE antibodies (Abs) were used as immunomagnetic capture probes. IgE Abs were extracted from a patient’s blood serum by the formation of a sandwich structure between the AuNPs and MBs. Multiple specific IgE Abs were simultaneously identified by mass spectrometry detection of the mass barcodes, providing an efficient component-resolved allergy diagnosis. Because of the signal amplification provided by the mass barcodes, the developed diagnosis method is very sensitive, with a limit of detection down to picograms per milliliter level for specific IgE Abs. The method can be potentially useful when the sample amount is highly limited and a multiplex diagnostic procedure is required.
000219128 700__ $$0247546$$g234678$$aZhong, Xiaoqin
000219128 700__ $$0242749$$g192106$$aQiao, Liang
000219128 700__ $$0244747$$g207324$$aGasilova, Natalia
000219128 700__ $$aLiu, Baohong
000219128 700__ $$0242739$$g105258$$aGirault, Hubert H.
000219128 773__ $$j88$$tAnalytical Chemistry$$k12$$q6184-6189
000219128 8564_ $$uhttps://infoscience.epfl.ch/record/219128/files/ac-2016-011428_0006.gif$$zTOC$$s12956$$yTOC
000219128 8564_ $$uhttps://infoscience.epfl.ch/record/219128/files/ac6b01142_si_001.pdf$$zSupporting Information$$s4082132$$ySupporting Information
000219128 8564_ $$uhttps://infoscience.epfl.ch/record/219128/files/acs2Eanalchem2E6b01142.pdf$$zPublisher's version$$s1130927$$yPublisher's version
000219128 909C0 $$xU10100$$0252090$$pLEPA
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000219128 917Z8 $$x208650
000219128 937__ $$aEPFL-ARTICLE-219128
000219128 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000219128 980__ $$aARTICLE