000218982 001__ 218982
000218982 005__ 20180317092343.0
000218982 037__ $$aPOST_TALK
000218982 245__ $$aBiostimulation as a remediation strategy post uranium acidic in situ recovery
000218982 269__ $$a2016
000218982 260__ $$c2016
000218982 336__ $$aPosters
000218982 520__ $$aA large fraction (47%) of the world’s uranium is mined by a technique called “In Situ Recovery”. This mining technique involves the injection of a leaching fluid (acid or alkaline) into a uranium-bearing aquifer and the pumping of the resulting solution through cation exchange columns for the recovery of the dissolved metal. A strategy of rehabilitation of the aquifer is based on the biostimulation of the autochthonous microbial community to remediate the acid plume and immobilize remaining trace metals. Assessment of the biostimulation was carried out using column tests filled with aquifer material. These columns were stimulated with a mixture of molasses, yeast extract and glycerol. Results showed that this mixture efficiently promoted the development of acid-resistant, sulfate-reducing bacterial guild members down to an inlet pH value ~ 4.7, with pH values at the outlet reaching up to 6.5 – 7. The rise of pH values along the columns were attributed to the predominant reduction of nitrate and sulfate. Biostimulation of the microbial community efficiently promoted the complete immobilization of U in the first centimeters of the columns. Synchrotron x-ray analysis and electron microscopy revealed that up to 60% of total U was precipitated as UIV onto bacterial cells. A detailed metagenomic analysis and qPCR data illustrated drastic changes occurring within the community with the reduction of the microbial diversity and the quantitative development of fermentative bacteria. The community was dominated by members of the Phylum Firmicutes (genus “Clostridium”, Pelosinus and Desulfosporosinus), representing up to 89 % of all cells.
000218982 700__ $$0249248$$aCoral, Thomas$$g189461
000218982 700__ $$0246837$$aReinsch, Brian Carl$$g229781
000218982 700__ $$0240018$$aBernier-Latmani, Rizlan$$g171550
000218982 700__ $$aDescostes, Michaël
000218982 700__ $$aDe Boissezon, Hélène
000218982 700__ $$0242691$$aRossi, Pierre$$g168011
000218982 7112_ $$aISME 16$$cMontréal, Canada$$dAugust 21-26, 2016
000218982 909CO $$ooai:infoscience.tind.io:218982$$pENAC$$pposter
000218982 909C0 $$0252083$$pGR-CEL$$xU11838
000218982 917Z8 $$x168011
000218982 937__ $$aEPFL-POSTER-218982
000218982 973__ $$aEPFL
000218982 980__ $$aPOSTER