000218844 001__ 218844
000218844 005__ 20190317000453.0
000218844 0247_ $$2doi$$a10.1039/c6ra01664b
000218844 022__ $$a2046-2069
000218844 02470 $$2ISI$$a000374974300115
000218844 037__ $$aARTICLE
000218844 245__ $$aPlatinum nanopetal-based potassium sensors for acute cell death monitoring
000218844 269__ $$a2016
000218844 260__ $$bRSC Publishing$$c2016$$aCambridge
000218844 300__ $$a10
000218844 336__ $$aJournal Articles
000218844 520__ $$aGrowing interest in the role of ions as cell death regulators has led to the consideration of K<sup>+</sup>, which plays a major role in events such as necrosis, apoptosis and osmotic shock. These mechanisms induce effluxes of K<sup>+</sup>, which can be measured to monitor such cellular events. In this work, we present a fast and simple template-free electrodeposition method for modifying electrodes on microfabricated Si-based platforms with Pt nanopetals. K<sup>+</sup>-selective electrodes were constructed by coupling such newly obtained Pt nanopetals, which were used as a solid contact, with PVC (polyvinyl chloride) K<sup>+</sup>-selective membranes. The drift over time was reduced by three orders of magnitude from several mV h<sup>-1</sup> for bare electrodes to tens of &mu;V h<sup>-1</sup>when Pt nanopetals were used as an intermediate layer between the electrode and the selective membrane. The improved potential stability is comparable to the best values obtained by using solid-contact ion-selective electrodes based on other nanomaterials. The sensors exhibited near-Nernstian behavior and high selectivity for K<sup>+</sup>. By studying cell viability in relation to K<sup>+</sup> measurements, we established a new correlation between the level of ions and the cell viability upon exposure to both osmotic shock and treatment with acetaminophen. The present method for the continuous and non-invasive monitoring of cell death in a bioreactor has potential applications in various biomedical domains.
000218844 700__ $$0245317$$g198935$$aTaurino, Irene
000218844 700__ $$aMassa, Solange
000218844 700__ $$aSanzo, Gabriella
000218844 700__ $$aAleman, Julio
000218844 700__ $$aFlavia, Basilotta
000218844 700__ $$aShin, Su Ryon
000218844 700__ $$aZhang, Yu Shrike
000218844 700__ $$aDökmeci, Mehmet Remzi
000218844 700__ $$0240269$$g167918$$aDe Micheli, Giovanni
000218844 700__ $$0242413$$g182237$$aCarrara, Sandro
000218844 700__ $$aKhademhosseini, Ali
000218844 773__ $$j6$$tRSC Advances$$k46$$q40517-40526
000218844 8564_ $$uhttps://infoscience.epfl.ch/record/218844/files/c6ra01664b.pdf$$zn/a$$s1997166$$yn/a
000218844 909C0 $$xU11140$$0252283$$pLSI1
000218844 909CO $$particle$$ooai:infoscience.tind.io:218844$$qGLOBAL_SET$$pSTI$$pIC
000218844 917Z8 $$x112915
000218844 917Z8 $$x112915
000218844 937__ $$aEPFL-ARTICLE-218844
000218844 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000218844 980__ $$aARTICLE