000218140 001__ 218140
000218140 005__ 20190416220329.0
000218140 0247_ $$2doi$$a10.1039/C6AN00126B
000218140 022__ $$a1364-5528
000218140 02470 $$2ISI$$a000377225600031
000218140 037__ $$aARTICLE
000218140 245__ $$aAluminium Foil as Single-Use Substrate for MALDI-MS Fingerprinting of Different Melanoma Cell Lines
000218140 269__ $$a2016
000218140 260__ $$bRoyal Soc Chemistry$$c2016$$aCambridge
000218140 300__ $$a8
000218140 336__ $$aJournal Articles
000218140 520__ $$aHerein, we present the intact cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the fingerprinting of human melanoma cancer cell lines grown on aluminium foil. To perform the MALDI-MS assay, melanoma cells were cultured on a flat and thin foil, which was directly transferred to the target plate of MALDI-MS for analysis. The influence of a wide range of cell fixation protocols (i.e. formalin-based and alcohol-based methods) and MALDI matrices on the obtained characteristic spectra was investigated. For the optimization of the MALDI-MS protocol, the MS fingerprints of the melanoma WM-239 cell line with and without an overexpressed enhanced green fluorescent protein were employed. The fingerprints obtained from WM-239 cells grown on aluminum foil were compared with intact cell MALDI of cell pellet and presented higher sensitivity in high m/z range. The optimized protocol was subsequently applied to characterise melanoma cell lines derived from different cancer stages and allowed the identification of unique MS signals that can be used for the differentiation between the studied cell lines (i.e. molecular weight equal to 10.0 kDa and 26.1 kDa).
000218140 700__ $$0245816$$g217921$$aBondarenko, Alexandra
000218140 700__ $$0248531$$g244161$$aZhu, Yingdi
000218140 700__ $$0242749$$g192106$$aQiao, Liang
000218140 700__ $$0242741$$g176551$$aCortes-Salazar, Fernando
000218140 700__ $$aPick, Horst$$g110882$$0241004
000218140 700__ $$aGirault, Hubert$$g105258$$0242739
000218140 773__ $$j141$$tThe Analyst$$q3403-3410
000218140 8564_ $$uhttps://infoscience.epfl.ch/record/218140/files/c6an00126b.pdf$$zPublisher's version$$s2197785$$yPublisher's version
000218140 8564_ $$uhttps://infoscience.epfl.ch/record/218140/files/c6an00126b1.pdf$$zSupporting Information$$s1760420$$ySupporting Information
000218140 909C0 $$xU10100$$0252090$$pLEPA
000218140 909CO $$ooai:infoscience.tind.io:218140$$qGLOBAL_SET$$pSB$$particle
000218140 917Z8 $$x208650
000218140 917Z8 $$x208650
000218140 937__ $$aEPFL-ARTICLE-218140
000218140 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000218140 980__ $$aARTICLE