000217952 001__ 217952
000217952 005__ 20181203024227.0
000217952 0247_ $$2doi$$a10.1016/j.eml.2016.03.017
000217952 022__ $$a2352-4316
000217952 02470 $$2ISI$$a000395259300001
000217952 037__ $$aARTICLE
000217952 245__ $$aOptimization of thin-film highly-compliant elastomer sensors for contractility measurement of muscle cells
000217952 260__ $$bElsevier Science Bv$$c2016$$aAmsterdam
000217952 269__ $$a2016
000217952 300__ $$a10
000217952 336__ $$aJournal Articles
000217952 520__ $$aTest assays capable of providing quantitative characterization of the contraction of cardiac and smooth muscle cells are of great need for drug development and screening. Several methodologies have been proposed for achieving measurement of cell contractile stress or force, however almost all rely on optical methods to detect contraction. Recently, we proposed a test assay method based on the cell-induced deformation of thin-film, elastomeric, capacitive sensors. The method uses an electrical (capacitive) read-out enabling facile up-scaling to a large number of devices working in parallel for high-throughput measurements. We present here a model for the prediction and optimization of sensor performance. Our model shows the following trends: a) a cell region ratio of approximately 0.75 of the culture well radius produces the largest change in capacitance for a given cell contractile stress, b) the change in capacitance generated by cell contraction increases as the Young’s modulus, sensing layer thickness and electrode thicknesses of the sensor decrease, following an inverse relationship. A prototype device is fabricated and characterized in cell culture conditions. Mean standard deviations as lows as 0.2 pF are achieved (<0.05% of the initial sensor capacitance), representing a minimum detectable cell stress of 1.2 kPa, as predicted by our model. This sensitivity is sufficient to measure the contractile stress of smooth and cardiac muscle cell monolayers as reported in the literature.
000217952 6531_ $$aThin-film elastomer sensors
000217952 6531_ $$aMuscle cell contraction
000217952 6531_ $$aCell contraction assay
000217952 6531_ $$aDielectric elastomer sensors
000217952 700__ $$aAraromi, O.
000217952 700__ $$0246641$$g179472$$aPoulin, Alexandre
000217952 700__ $$0241188$$g127048$$aRosset, Samuel
000217952 700__ $$0249018$$g254884$$aImboden, Matthias
000217952 700__ $$aFavre, M.
000217952 700__ $$aGiazzon, M.
000217952 700__ $$aMartin-Olmos, C.
000217952 700__ $$g234179$$aSorba, Francesca$$0249077
000217952 700__ $$aLiley, M.
000217952 700__ $$0240376$$g162368$$aShea, Herbert
000217952 773__ $$j9$$tExtreme Mechanics Letters$$k1$$q1-10
000217952 909C0 $$xU10955$$0252107$$pLMTS
000217952 909CO $$ooai:infoscience.tind.io:217952$$qtest$$pSTI$$particle
000217952 917Z8 $$x162368
000217952 917Z8 $$x127048
000217952 937__ $$aEPFL-ARTICLE-217952
000217952 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000217952 980__ $$aARTICLE