000217734 001__ 217734
000217734 005__ 20181203024221.0
000217734 0247_ $$2doi$$a10.1371/journal.pone.0148644
000217734 022__ $$a1932-6203
000217734 02470 $$2ISI$$a000370050700024
000217734 037__ $$aARTICLE
000217734 245__ $$aNext-Generation Sequencing of Aquatic Oligochaetes: Comparison of Experimental Communities
000217734 260__ $$bPublic Library of Science$$c2016$$aSan Francisco
000217734 269__ $$a2016
000217734 300__ $$a14
000217734 336__ $$aJournal Articles
000217734 520__ $$aAquatic oligochaetes are a common group of freshwater benthic invertebrates known to be very sensitive to environmental changes and currently used as bioindicators in some countries. However, more extensive application of oligochaetes for assessing the ecological quality of sediments in watercourses and lakes would require overcoming the difficulties related to morphology-based identification of oligochaetes species. This study tested the Next-Generation Sequencing (NGS) of a standard cytochrome c oxydase I (COI) barcode as a tool for the rapid assessment of oligochaete diversity in environmental samples, based on mixed specimen samples. To know the composition of each sample we Sanger sequenced every specimen present in these samples. Our study showed that a large majority of OTUs (Operational Taxonomic Unit) could be detected by NGS analyses. We also observed congruence between the NGS and specimen abundance data for several but not all OTUs. Because the differences in sequence abundance data were consistent across samples, we exploited these variations to empirically design correction factors. We showed that such factors increased the congruence between the values of oligochaetes-based indices inferred from the NGS and the Sanger-sequenced specimen data. The validation of these correction factors by further experimental studies will be needed for the adaptation and use of NGS technology in biomonitoring studies based on oligochaete communities.
000217734 700__ $$uEawag EPFL, Ecotox Ctr, Swiss Ctr Appl Ecotoxicol, CH-1015 Lausanne, Switzerland$$aVivien, Regis
000217734 700__ $$aLejzerowicz, Franck
000217734 700__ $$aPawlowski, Jan
000217734 773__ $$j11$$tPlos One$$k2$$qe0148644
000217734 909C0 $$xUS18204$$0252506$$pECOTOX
000217734 909CO $$particle$$pENAC$$ooai:infoscience.tind.io:217734
000217734 917Z8 $$x242289
000217734 937__ $$aEPFL-ARTICLE-217734
000217734 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000217734 980__ $$aARTICLE