Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus Optical Coherence Microscopy

We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus configuration of OCM instrument involving Bessel beam. It provided access to detailed 3D information about the changes in brain vascular system up to the level of capillaries across I and II/III layers of neocortex. We used photothrombotic stroke model involving photoactive application of rose bengal to assure minimal invasiveness of the procedure and precise localization of the clot distribution center. We present the comparative analysis involving structural and angiographic maps of the stroke-affected brain enabling in-depth insight to the process of development of the disorder.

Published in:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 969718
Presented at:
SPIE Photonics West, BiOS, San Francisco, January 13-18, 2016
SPIE digital Library

 Record created 2016-03-24, last modified 2018-09-13

Publisher's version:
Download fulltext

Rate this document:

Rate this document:
(Not yet reviewed)