000216037 001__ 216037
000216037 005__ 20181203024143.0
000216037 0247_ $$2doi$$a10.1242/bio.011957
000216037 022__ $$a2046-6390
000216037 02470 $$2ISI$$a000365076600009
000216037 037__ $$aARTICLE
000216037 245__ $$aFlagellar membranes are rich in raft-forming phospholipids
000216037 260__ $$bCompany Of Biologists Ltd$$c2015$$aCambridge
000216037 269__ $$a2015
000216037 300__ $$a11
000216037 336__ $$aJournal Articles
000216037 520__ $$aThe observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei.
000216037 6531_ $$aFlagella
000216037 6531_ $$aLipid rafts
000216037 6531_ $$aMembrane lipids
000216037 6531_ $$aMass spectrometry
000216037 6531_ $$aSphingolipids
000216037 6531_ $$aTrypanosoma brucei
000216037 700__ $$uUniv Bern, Inst Biochem & Mol Med, CH-3012 Bern, Switzerland$$aSerricchio, Mauro
000216037 700__ $$uEcole Polytech Fed Lausanne, Prote Core Facil, CH-1015 Lausanne, Switzerland$$aSchmid, Adrien W.
000216037 700__ $$uUniv Bern, Inst Biochem & Mol Med, CH-3012 Bern, Switzerland$$aSteinmann, Michael E.
000216037 700__ $$uUniv Bern, Inst Biochem & Mol Med, CH-3012 Bern, Switzerland$$aSigel, Erwin
000216037 700__ $$uUniv Bern, Inst Biochem & Mol Med, CH-3012 Bern, Switzerland$$aRauch, Monika
000216037 700__ $$uInst Pasteur, Trypanosome Cell Biol Unit, F-75015 Paris, France$$aJulkowska, Daria
000216037 700__ $$uInst Pasteur, Trypanosome Cell Biol Unit, F-75015 Paris, France$$aBonnefoy, Serge
000216037 700__ $$uInst Pasteur, Trypanosome Cell Biol Unit, F-75015 Paris, France$$aFort, Cecile
000216037 700__ $$uInst Pasteur, Trypanosome Cell Biol Unit, F-75015 Paris, France$$aBastin, Philippe
000216037 700__ $$uUniv Bern, Inst Biochem & Mol Med, CH-3012 Bern, Switzerland$$aBuetikofer, Peter
000216037 773__ $$j4$$tBiology Open$$k9$$q1143-1153
000216037 909C0 $$0252559$$pENT$$xU12833
000216037 909CO $$particle$$ooai:infoscience.tind.io:216037
000216037 937__ $$aEPFL-ARTICLE-216037
000216037 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000216037 980__ $$aARTICLE