Sequencing by Hybridization of Long Targets

Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.

Editor(s):
Gibas, Cynthia
Published in:
PLoS One, 7, 5, e35819
Year:
2012
ISSN:
1932-6203
ISBN:
1932-6203 (Electronic)$backslash$r1932-6203 (Linking)
Keywords:
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