Sequencing by Hybridization of Long Targets

Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.


Editor(s):
Gibas, Cynthia
Published in:
PLoS One, 7, 5, e35819
Year:
2012
ISSN:
1932-6203
Keywords:
Laboratories:




 Record created 2015-12-07, last modified 2018-09-13

External link:
Download fulltext
URL
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)