Sequencing by Hybridization of Long Targets
Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.
- URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3344849&tool=pmcentrez&rendertype=abstract http://dx.plos.org/10.1371/journal.pone.0035819
Keywords: DNA ; DNA Probes ; DNA Probes: genetics ; DNA: genetics ; DNA: methods ; Nucleic Acid ; Nucleic Acid Hybridization ; Nucleic Acid Hybridization: methods ; Repetitive Sequences ; Sequence Analysis
Record created on 2015-12-07, modified on 2016-08-09