000213983 001__ 213983
000213983 005__ 20190617200734.0
000213983 0247_ $$2doi$$a10.1016/j.cpc.2015.06.024
000213983 022__ $$a0010-4655
000213983 02470 $$2ISI$$a000362602900053
000213983 037__ $$aARTICLE
000213983 245__ $$aCalibration of optical tweezers with non-spherical probes via high-resolution detection of Brownian motion
000213983 260__ $$bElsevier Science Bv$$c2015$$aAmsterdam
000213983 269__ $$a2015
000213983 300__ $$a12
000213983 336__ $$aJournal Articles
000213983 520__ $$aOptical tweezers are commonly used and powerful tools to perform force measurements on the piconewton scale and to detect nanometer-scaled displacements. However, the precision of these instruments relies to a great extent on the accuracy of the calibration method. A well-known calibration procedure is to record the stochastic motion of the trapped particle and compare its statistical behavior with the theory of the Brownian motion in a harmonic potential. Here we present an interactive calibration software which allows for the simultaneous fitting of three different statistical observables (power spectral density, mean square displacement and velocity autocorrelation function) calculated from the trajectory of the probe to enhance fitting accuracy. The fitted theory involves the hydrodynamic interactions experimentally observable at high sampling rates. Furthermore, a qualitative extension is included in our model to handle the thermal fluctuations in the orientation of optically trapped asymmetric objects. The presented calibration methodology requires no prior knowledge of the bead size and can be applied to non-spherical probes as well. The software was validated on synthetic and experimental data. Program summary Program title: PFMCal Catalogue identifier: AEXH_v1_0 Program summary URL: http://cpc.cs.qub.ac.ukisummaries/AEXH_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 206,399 No. of bytes in distributed program, including test data, etc.: 10,319,465 Distribution format: tar.gz Programming language: MatLab 2011a (Math Works Inc.). Computer: General computer running MatLab (MathWorks Inc.), using Statistics Toolbox. Operating system: Any which supports Matlab using Statistics Toolbox. RAM: 10 MB Classification: 3, 4.9, 18, 23. Nature of problem: Calibration of optical tweezers by measuring the Brownian motion of the trapped object. The voltage-to-displacement ratio of the detection system (the inverse of the sensitivity), the stiffness of the trap and the size of the bead are obtained via the simultaneous fitting of the power spectral density (PSD), mean square displacement (MSD) and velocity autocorrelation (VAF) functions calculated from the trajectory. The calibration can be performed for non-spherical probes as well. Solution method: Initialization points for all parameters are inferred from characteristic features of the statistical observables (PSD, MSD and VAF) based on the method developed by Grimm et al. in [1]. Theoretical functions for the PSD, the MSD and the VAF are calculated from the model of Brownian motion confined by a harmonic potential taking hydrodynamic interactions into consideration [2-4]. This calibration methodology has been successfully used in actual experiments with micro-spheres [5, 6]. Calculated functions are fitted to the measurement data via the Levenberg-Marquardt least square fitting routine available in the MatLab Optimalization Toolbox, using the nlinfit function. If the error to the measured data has been estimated, the corresponding data values can be weighted by the inverse of the standard error squared in order to eliminate bias introduced by heteroscedasticity. In order to increase robustness and avoid convergence to local minima, minimum search from multiple initial values in the vicinity of the first guess is possible. Additional comments: Input of the program is the experimental PSDexp, MSDexp, and VAF(exp) data points calculated from the measured x, y and z projections of the trajectory of the particle. The data may best be blocked and may optionally contain an error for each data point for better results. The data should be formatted into three columns: 1. independent variables (time or frequency array), 2. function values, 3. optionally, error values (e.g. standard error calculated from the binning) to improve fitting efficiency. In case error values were not estimated, the third column should be filled with zeros. The first row is reserved for a header, data is read from the second row. Data size should not be larger than a few hundreds of rows, otherwise, using larger blocks for binning is advised. In order to observe the short time behavior of the Brownian motion, influenced by hydrodynamic effects, the sampling rate should be typically higher than 100 kHz. Total sampling time is typically tens of seconds, to achieve a good resolution in the frequency range. The optical axis of the laser, the microscope and the detector system should be co-aligned to exclude artificial crosstalk between the x, y and z channels of the position detector. Running time: Seconds (C) 2015 Elsevier B.V. All rights reserved.
000213983 6531_ $$aCalibration of optical tweezers
000213983 6531_ $$aNon-spherical probe
000213983 6531_ $$aBrownian motion
000213983 6531_ $$aPower spectral density (PSD)
000213983 6531_ $$aMean square displacement (MSD)
000213983 6531_ $$aVelocity autocorrelation function (VAF)
000213983 700__ $$uBudapest Univ Technol & Econ, Dept Phys, H-1111 Budapest, Hungary$$aButykai, A.
000213983 700__ $$0244820$$g168736$$uEcole Polytech Fed Lausanne, Lab Phys Complex Matter, CH-1015 Lausanne, Switzerland$$aMor, F. M.
000213983 700__ $$0240362$$g124105$$uEcole Polytech Fed Lausanne, Lab Phys Complex Matter, CH-1015 Lausanne, Switzerland$$aGaal, R.
000213983 700__ $$aDominguez-Garcia, P.
000213983 700__ $$0240227$$g105148$$uEcole Polytech Fed Lausanne, Lab Phys Complex Matter, CH-1015 Lausanne, Switzerland$$aForro, L.
000213983 700__ $$aJeney, S.$$uEcole Polytech Fed Lausanne, Lab Phys Complex Matter, CH-1015 Lausanne, Switzerland$$g158461$$0240917
000213983 773__ $$j196$$tComputer Physics Communications$$q599-610
000213983 909C0 $$xU10142$$0252321$$pLPMC
000213983 909CO $$ooai:infoscience.tind.io:213983$$qGLOBAL_SET$$pSB$$particle
000213983 917Z8 $$x240662
000213983 937__ $$aEPFL-ARTICLE-213983
000213983 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000213983 980__ $$aARTICLE