Electrophysiological imaging of epileptic brain slices reveals pharmacologically confined functional changes

Microelectrode arrays (MEAs) are employed to study extracellular electrical activity in neuronal tissues. Neverthe- less, commercially available MEAs provide a limited number of recording sites and do not allow a precise identifi- cation of the spatio-temporal characterization of the recorded signal. To overcome this limitation, high density MEAs, based on CMOS technology, were recently developed and validated on dissociated preparations (Ber- dondini et al. 2009). We show the platform capability to record extracellular electrophysiological signal from 4096 electrodes arranged in a squared area of 2.7 mm x 2.7 mm with inter-electrode distance of 21 μm at a sampling rate of 7.7 kHz/electrode. Here, we demonstrate the performances of these platforms for the acquisition chemi- cally evoked epileptiform activity from brain slices. Moreover the high spatial resolutions allow us to estimate the effect of drugs in spatially modulating Inter-Ictal ((I-IC) activity.


Published in:
Proceedings of the 8th International Meeting on Substrate-Integrated Microelectrode Arrays
Presented at:
8th International Meeting on Substrate-Integrated Microelectrode Arrays (SIMEA), Reutlingen, Germany, July 10-13, 2012
Year:
2012
Laboratories:




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