000213577 001__ 213577
000213577 005__ 20190415234931.0
000213577 0247_ $$2doi$$a10.1007/978-1-4939-2510-0_12
000213577 020__ $$a978-1-4939-2509-4
000213577 037__ $$aBOOK_CHAP
000213577 245__ $$aCompartmentalized Microfluidics for In Vitro Alzheimer’s Disease Studies
000213577 269__ $$a2015
000213577 260__ $$bSpringer New York$$c2015$$aNew York, NY
000213577 336__ $$aBook Chapters
000213577 490__ $$aNeuromethods$$v103
000213577 520__ $$aCompartmentalized microfluidic devices are designed to engineer the cellular environment for cell cultures. The practical use of the compartmentalized chambers can be expanded to induce co-pathological cell cultures, where one cell population expresses a specific disease state, while being in direct-cell or metabolic contact to a second or third unaffected cell population. A typical example for co-pathological cell states in the brain is the well-known neurodegenerative Alzheimer’s disease (AD), which still lacks effective treatment approaches. In the brain, AD shows specific disease progression patterns from one functional brain region to another. However, in normal dissociated neuron cultures using petri dishes, the extraction of the progression patterns is very difficult. In this chapter, we describe the methodology to design and fabricate a compartmentalized microfluidic device and apply it to an in vitro AD model to mimic the key pathological hallmarks of AD, allowing us to study disease progression patterns from a “diseased” towards a “healthy” cell population. This derived co-pathological model of AD provides the ability to monitor time-variant changes in cell network morphology and electrophysiology during disease progression and may potentially be used for pharmaceutical tests.
000213577 6531_ $$aAlzheimer’s disease
000213577 6531_ $$aCo-pathology
000213577 6531_ $$aDisease progression
000213577 6531_ $$aCompartmentalized microfluidic device
000213577 6531_ $$aMicro-electrode arrays (MEAs)
000213577 6531_ $$aNeural cell culture
000213577 700__ $$0245911$$g200339$$aRen, Yufei
000213577 700__ $$0242554$$g168829$$aKunze, Anja
000213577 700__ $$0240219$$g107144$$aRenaud, Philippe
000213577 720_1 $$aBiffi, Emilia$$eed.
000213577 773__ $$tMicrofluidic and Compartmentalized Platforms for Neurobiological Research$$q197-215
000213577 8564_ $$uhttps://infoscience.epfl.ch/record/213577/files/Ren2015.pdf$$zn/a$$s2089329$$yn/a
000213577 909C0 $$xU10324$$0252064$$pLMIS4
000213577 909CO $$ooai:infoscience.tind.io:213577$$pSTI$$pbook$$pchapter$$qGLOBAL_SET
000213577 917Z8 $$x113143
000213577 937__ $$aEPFL-CHAPTER-213577
000213577 973__ $$sPUBLISHED$$aEPFL
000213577 980__ $$aCHAPTER