Hyperpolarized Water to Study Protein-Ligand Interactions

The affinity between a chosen target protein and small molecules is a key aspect of drug discovery. Screening by popular NMR methods such as Water-LOGSY suffers from low sensitivity and from false positives caused by aggregated or denatured proteins. This work demonstrates that the sensitivity of Water-LOGSY can be greatly boosted by injecting hyperpolarized water into solutions of proteins and ligands. Ligand binding can be detected in a few seconds, whereas about 30 min is usually required without hyperpolarization. Hyperpolarized water also enhances proton signals of proteins at concentrations below 20 M so that one can verify in a few seconds whether the proteins remain intact or have been denatured.


Published in:
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 6, 9, 1674-1678
Year:
2015
ISSN:
1948-7185
Laboratories:




 Record created 2015-11-10, last modified 2018-09-13

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P391a-Supp-Chappuis-Milani-Vuichoud-Bornet-Gossert-Bodenhausen –Jannin-Hyperpolarized-LOGSY- JPCL-6-1674−2015 - Download fulltextPDF
P391-Chappuis-Milani-Vuichoud-Bornet-Gossert-Bodenhausen –Jannin-Hyperpolarized-LOGSY- JPCL-6-1674−2015 - Download fulltextPDF
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