000212366 001__ 212366
000212366 005__ 20180913063345.0
000212366 0247_ $$2doi$$a10.1039/c5nr01355k
000212366 022__ $$a2040-3364
000212366 02470 $$2ISI$$a000356862900023
000212366 037__ $$aARTICLE
000212366 245__ $$aInfluence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes
000212366 269__ $$a2015
000212366 260__ $$aCambridge$$bRoyal Society of Chemistry$$c2015
000212366 300__ $$a13
000212366 336__ $$aJournal Articles
000212366 520__ $$aErythrocytes are attractive as potential cell-based drug carriers because of their abundance and long life-span in vivo. Existing methods for loading drug cargos into erythrocytes include hypotonic treatments, electroporation, and covalent attachment onto the membrane, all of which require ex vivo manipulation. Here, we characterized the properties of amphiphilic gold nanoparticles (amph-AuNPs), comprised of a similar to 2.3 nm gold core and an amphiphilic ligand shell, which are able to embed spontaneously within erythrocyte membranes and might provide a means to load drugs into red blood cells (RBCs) directly in vivo. Particle interaction with RBC membranes occurred rapidly at physiological temperature. We further show that amph-AuNP uptake by RBCs was limited by the glycocalyx and was particularly influenced by sialic acids on cell surface proteoglycans. Using a reductionist model membrane system with synthetic lipid vesicles, we confirmed the importance of membrane fluidity and the glycocalyx in regulating amph-AuNP/ membrane interactions. These results thus provide evidence for the interaction of amph-AuNPs with erythrocyte membranes and identify key membrane components that govern this interaction, providing a framework for the development of amph-AuNP-carrying erythrocyte 'pharmacytes' in vivo.
000212366 700__ $$aAtukorale, Prabhani U.$$uMIT, Dept Biol Engn, Cambridge, MA 01239 USA
000212366 700__ $$aYang, Yu-Sang$$uMIT, Dept Mat Sci & Engn, Cambridge, MA 01239 USA
000212366 700__ $$0247324$$aBekdemir, Ahmet$$g233468$$uEcole Polytech Fed Lausanne, Inst Mat, CH-1015 Lausanne, Switzerland
000212366 700__ $$aCarney, Randy P.$$uEcole Polytech Fed Lausanne, Inst Mat, CH-1015 Lausanne, Switzerland
000212366 700__ $$aSilva, Paulo J.$$uEcole Polytech Fed Lausanne, Inst Mat, CH-1015 Lausanne, Switzerland
000212366 700__ $$aWatson, Nicki$$uWhitehead Inst Biomed Res, Cambridge, MA 01239 USA
000212366 700__ $$0244724$$aStellacci, Francesco$$g196998$$uEcole Polytech Fed Lausanne, Inst Mat, CH-1015 Lausanne, Switzerland
000212366 700__ $$aIrvine, Darrell J.$$uMIT, Dept Biol Engn, Cambridge, MA 01239 USA
000212366 773__ $$j7$$k26$$q11420-11432$$tNanoscale
000212366 8564_ $$s4044284$$uhttps://infoscience.epfl.ch/record/212366/files/Atukorale%20et%20al.%20-%202015%20-%20Influence%20of%20the%20glycocalyx%20and%20plasma%20membrane%20co.pdf$$yn/a$$zn/a
000212366 909C0 $$0252323$$pSUNMIL$$xU12173
000212366 909CO $$ooai:infoscience.tind.io:212366$$pSTI$$particle
000212366 917Z8 $$x196998
000212366 937__ $$aEPFL-ARTICLE-212366
000212366 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000212366 980__ $$aARTICLE