000212125 001__ 212125
000212125 005__ 20190213064548.0
000212125 0247_ $$2doi$$a10.1038/Nmeth.3442
000212125 022__ $$a1548-7091
000212125 02470 $$2ISI$$a000358736100012
000212125 037__ $$aARTICLE
000212125 245__ $$aQuantitative evaluation of software packages for single-molecule localization microscopy
000212125 260__ $$bNature Publishing Group$$c2015$$aLondon
000212125 269__ $$a2015
000212125 300__ $$a12
000212125 336__ $$aJournal Articles
000212125 520__ $$9eng$$a The quality of super-resolution images obtained by single-molecule localization microscopy (SMLM) depends largely on the software used to detect and accurately localize point sources. In this work, we focus on the computational aspects of super-resolution microscopy and present a comprehensive evaluation of localization software packages. Our philosophy is to evaluate each package as a whole, thus maintaining the integrity of the software. We prepared synthetic data that represent three-dimensional structures modeled after biological components, taking excitation parameters, noise sources, point-spread functions and pixelation into account. We then asked developers to run their software on our data; most responded favorably, allowing us to present a broad picture of the methods available. We evaluated their results using quantitative and user-interpretable criteria: detection rate, accuracy, quality of image reconstruction, resolution, software usability and computational resources. These metrics reflect the various tradeoffs of SMLM software packages and help users to choose the software that fits their needs.
000212125 700__ $$0240181$$g115533$$uEcole Polytech Fed Lausanne, Biomed Imaging Grp, CH-1015 Lausanne, Switzerland$$aSage, Daniel
000212125 700__ $$0242489$$g194014$$uEcole Polytech Fed Lausanne, Biomed Imaging Grp, CH-1015 Lausanne, Switzerland$$aKirshner, Hagai
000212125 700__ $$uCtr Genom Regulat, Barcelona, Spain$$aPengo, Thomas
000212125 700__ $$uUniv Calif UCSF, Howard Hughes Med Inst, San Francisco, CA USA$$aStuurman, Nico
000212125 700__ $$uKorea Adv Inst Sci & Technol, Dept Bio & Brain Engn, Taejon 305701, South Korea$$aMin, Junhong
000212125 700__ $$aManley, Suliana
000212125 700__ $$aUnser, Michael$$uEcole Polytech Fed Lausanne, Biomed Imaging Grp, CH-1015 Lausanne, Switzerland$$g115227$$0240182
000212125 773__ $$j12$$tNature Methods$$k8$$q717-U37
000212125 8564_ $$uhttp://bigwww.epfl.ch/publications/sage1501.html$$zURL
000212125 8564_ $$uhttp://bigwww.epfl.ch/publications/sage1501.pdf$$zURL
000212125 8564_ $$uhttp://bigwww.epfl.ch/publications/sage1501.ps$$zURL
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000212125 937__ $$aEPFL-ARTICLE-212125
000212125 970__ $$asage1501/LIB
000212125 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000212125 980__ $$aARTICLE