Methods using o<6>-alkylguanine-dna alkyltransferases

A method using O<6>-alkylguanine-DNA alkyltransferases (AGT) is disclosed for transferring a label from a substrate to a fusion protein comprising the AGT. This allows the detection and/or manipulating of the fusion protein, both in vitro and in vivo, by attaching molecules to the fusion proteins that introduce a new physical or chemical property to the fusion protein. Examples of such molecules are, among others, spectroscopic probes or reporter molecules, affinity tags, molecules generating reactive radicals, cross-linkers, ligands mediating protein-protein interactions or molecules suitable for the immobilisation of the fusion protein.


Year:
2004
Other identifiers:
TTO: 6.0310
EPO Family ID: 23083030
Laboratories:




 Record created 2015-09-22, last modified 2018-03-18


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