000210323 001__ 210323
000210323 005__ 20190528082006.0
000210323 0247_ $$2doi$$a10.1371/journal.pone.0131037
000210323 022__ $$a1932-6203
000210323 02470 $$2ISI$$a000356933800111
000210323 037__ $$aARTICLE
000210323 245__ $$aDevelopment of a Bioluminescent Nitroreductase Probe for Preclinical Imaging
000210323 260__ $$bPublic Library of Science$$c2015$$aSan Francisco
000210323 269__ $$a2015
000210323 300__ $$a18
000210323 336__ $$aJournal Articles
000210323 520__ $$aBacterial nitroreductases (NTRs) have been widely utilized in the development of novel antibiotics, degradation of pollutants, and gene-directed enzyme prodrug therapy (GDEPT) of cancer that reached clinical trials. In case of GDEPT, since NTR is not naturally present in mammalian cells, the prodrug is activated selectively in NTR-transformed cancer cells, allowing high efficiency treatment of tumors. Currently, no bioluminescent probes exist for sensitive, non-invasive imaging of NTR expression. We therefore developed a "NTR caged luciferin" (NCL) probe that is selectively reduced by NTR, producing light proportional to the NTR activity. Here we report successful application of this probe for imaging of NTR in vitro, in bacteria and cancer cells, as well as in vivo in mouse models of bacterial infection and NTR-expressing tumor xenografts. This novel tool should significantly accelerate the development of cancer therapy approaches based on GDEPT and other fields where NTR expression is important.
000210323 700__ $$0246664$$g225303$$aVorobyeva, Anzhelika G
000210323 700__ $$aStanton, Michael
000210323 700__ $$0246450$$g166900$$aGodinat, Aurélien
000210323 700__ $$aLund, Kjetil B
000210323 700__ $$0246699$$g225224$$aKarateev, Grigory G
000210323 700__ $$aFrancis, Kevin P
000210323 700__ $$aAllen, Elizabeth
000210323 700__ $$aGelovani, Juri G
000210323 700__ $$aMcCormack, Emmet
000210323 700__ $$aTangney, Mark
000210323 700__ $$aDubikovskaya, Elena A
000210323 773__ $$j10$$tPloS One$$k6$$qe0131037
000210323 909C0 $$xU12499$$0252473$$pLCBIM
000210323 909CO $$pSB$$particle$$ooai:infoscience.tind.io:210323
000210323 917Z8 $$x225298
000210323 937__ $$aEPFL-ARTICLE-210323
000210323 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000210323 980__ $$aARTICLE