000208937 001__ 208937
000208937 005__ 20180317093905.0
000208937 022__ $$a2156-7085
000208937 02470 $$2ISI$$a000357435200006
000208937 0247_ $$2doi$$a10.1364/BOE.6.002353
000208937 037__ $$aARTICLE
000208937 245__ $$aMultiscattering-enhanced optical biosensor: multiplexed, non-invasive and continuous measurements of cellular processes
000208937 269__ $$a2015
000208937 260__ $$aWashington$$bOptical Soc Amer$$c2015
000208937 300__ $$a13
000208937 336__ $$aJournal Articles
000208937 520__ $$aThe continuous measurement of uptake or release of biomarkers provides invaluable information for understanding and monitoring the metabolism of cells. In this work, a multiscattering-enhanced optical biosensor for the multiplexed, non-invasive, and continuous detection of hydrogen peroxide (H2O2), lactate and glucose is presented. The sensing scheme is based on optical monitoring of the oxidation state of the metalloprotein cytochrome c (cyt c). The analyte of interest is enzymatically converted into H2O2 leading to an oxidation of the cyt c. Contact microspotting is used to prepare nanoliter-sized sensing spots containing either pure cyt c, a mixture of cyt c with glucose oxidase (GOx) to detect glucose, or a mixture of cyt c with lactate oxidase (LOx) to detect lactate. The sensing spots are embedded in a multiscattering porous medium that enhances the optical signal. We achieve limits of detection down to 240 nM and 110 nM for lactate and glucose, respectively. A microfluidic embodiment enables multiplexed and crosstalk-free experiments on living organisms. As an example, we study the uptake of exogenously supplied glucose by the green algae Chlamydomonas reinhardtii and simultaneously monitor the stress-related generation of H2O2. This multifunctional detection scheme provides a powerful tool to study biochemical processes at cellular level.
000208937 6531_ $$aNanophotonics
000208937 6531_ $$aPlasmonics
000208937 700__ $$0245839$$aKoman, Volodymyr$$g214827
000208937 700__ $$0240847$$aSantschi, Christian$$g117095
000208937 700__ $$aMartin, Olivier J.F.
000208937 773__ $$j6$$q2353-2365$$tBiomedical Optics Express
000208937 909CO $$ooai:infoscience.tind.io:208937$$particle$$pSTI
000208937 909C0 $$0252353$$pNAM$$xU10373
000208937 917Z8 $$x229344
000208937 937__ $$aEPFL-ARTICLE-208937
000208937 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000208937 980__ $$aARTICLE