000208488 001__ 208488
000208488 005__ 20180913063129.0
000208488 0247_ $$2doi$$a10.1016/j.virol.2014.11.002
000208488 022__ $$a0042-6822
000208488 02470 $$2ISI$$a000351484600016
000208488 037__ $$aARTICLE
000208488 245__ $$aStructure and biochemical characterization of bacteriophage phi92 endosialidase
000208488 260__ $$aSan Diego$$bAcademic Press Inc Elsevier Science$$c2015
000208488 269__ $$a2015
000208488 300__ $$a11
000208488 336__ $$aJournal Articles
000208488 520__ $$aSurface-associated capsular polysaccharides (CPSs) protect bacteria against phage infection and enhance pathogenicity by interfering with the function of the host innate immune system. The CPS of enteropathogenic Escherichia coli K92 is a unique sialic acid polymer (polySia) with alternating alpha 2,8- and alpha 2,9-linkages. This CPS can be digested by the gene 143 encoded endosialidase of bacteriophage phi92. Here we report the crystal structure of the phi92 endosialidase in complex with a dimer of alpha 2,9-linked sialic acid and analyze its catalytic functions. Unlike the well characterized and homologous endosialidase of phage K1F, the phi92 endosialidase is a bifunctional enzyme with high activity against alpha 2,8- and low activity against alpha 2,9-linkages in a polySia chain. Moreover, in contrast to the processive K1F endosialidase, the phi92 endosialidase degrades the polymer in a non-processive mode. Beyond describing the first endosialidase with alpha 2,9-specificity, our data introduce a novel platform for studies of endosialidase regioselectivity and for engineering highly active alpha 2,9-specific enzymes. (C) 2014 Elsevier Inc. All rights reserved.
000208488 6531_ $$aEndosialidase
000208488 6531_ $$aalpha 2
000208488 6531_ $$a9-linked polysialic acid
000208488 6531_ $$aCrystal structure
000208488 6531_ $$aEndo-alpha 2
000208488 6531_ $$a9-sialidase
000208488 6531_ $$aPhi92 bacteriophage
000208488 700__ $$0243842$$aSchwarzer, David$$g196709$$uHannover Med Sch MHH, Inst Cellular Chem, D-30625 Hannover, Germany
000208488 700__ $$0243841$$aBrowning, Christopher$$g190195
000208488 700__ $$aStummeyer, Katharina$$uHannover Med Sch MHH, Inst Cellular Chem, D-30625 Hannover, Germany
000208488 700__ $$aOberbeck, Astrid$$uHannover Med Sch MHH, Inst Cellular Chem, D-30625 Hannover, Germany
000208488 700__ $$aMuehlenhoff, Martina$$uHannover Med Sch MHH, Inst Cellular Chem, D-30625 Hannover, Germany
000208488 700__ $$aGerardy-Schahn, Rita$$uHannover Med Sch MHH, Inst Cellular Chem, D-30625 Hannover, Germany
000208488 700__ $$0243845$$aLeiman, Petr G.$$g188499
000208488 773__ $$j477$$q133-143$$tVirology
000208488 909C0 $$0252291$$pLBBS$$xU11967
000208488 909CO $$ooai:infoscience.tind.io:208488$$pSB$$particle
000208488 917Z8 $$x188499
000208488 937__ $$aEPFL-ARTICLE-208488
000208488 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000208488 980__ $$aARTICLE