Lighting conditions in workplaces contribute to a variety of factors related to work satisfaction, productivity and well-being. We tested whether different photometric variables also influence visual perception and the comfort of the lighting, as well as subjective non-visual variables such as mood, alertness and well-being. Twenty-five young subjects spent two afternoons either under electric light or daylighting conditions (without view from the window). Subjects overall preferred the daylighting for visual acceptance and glare. Changes of photometric variables modulated changes in visual light perception, alertness and mood in the course of the afternoon. Finally, we found several associations of visual and non-visual functions, indicating a potential relationship of visual comfort with other circadian and wake-dependent functions in humans, which consequently could impact office lighting scenarios in the future.