000208281 001__ 208281
000208281 005__ 20180913063125.0
000208281 0247_ $$2doi$$a10.1007/s00441-014-2109-0
000208281 022__ $$a0302-766X
000208281 02470 $$2ISI$$a000352072500013
000208281 037__ $$aARTICLE
000208281 245__ $$aDiffraction-unlimited imaging: from pretty pictures to hard numbers
000208281 260__ $$aNew York$$bSpringer Verlag$$c2015
000208281 269__ $$a2015
000208281 300__ $$a28
000208281 336__ $$aReviews
000208281 520__ $$aDiffraction-unlimited fluorescence imaging allows the visualization of intact, strongly heterogeneous systems at unprecedented levels of detail. Beyond the acquisition of detailed pictures, increasing efforts are now being focused on deriving quantitative insights from these techniques. In this work, we review the recent developments on sub-diffraction quantization that have arisen for the various techniques currently in use. We pay particular attention to the information that can be obtained but also the practical problems that can be faced, and provide suggestions for solutions or workarounds. We also show that these quantitative metrics not only provide a way to turn raw data into hard statistics but also help to understand the features and pitfalls associated with sub-diffraction imaging. Ultimately, these developments will lead to a highly standardized and easily applicable toolbox of techniques, which will find widespread application in the scientific community.
000208281 6531_ $$aSuper-resolution
000208281 6531_ $$aFluorophores
000208281 6531_ $$aFluorescence
000208281 6531_ $$aQuantitative analysis
000208281 6531_ $$aDiffraction-unlimited
000208281 700__ $$aVandenberg, Wim$$uUniv Leuven, Dept Chem, B-3001 Heverlee, Belgium
000208281 700__ $$0240334$$aLeutenegger, Marcel$$g113715
000208281 700__ $$0240555$$aLasser, Theo$$g120900
000208281 700__ $$aHofkens, Johan$$uUniv Leuven, Dept Chem, B-3001 Heverlee, Belgium
000208281 700__ $$aDedecker, Peter$$uUniv Leuven, Dept Chem, B-3001 Heverlee, Belgium
000208281 773__ $$j360$$k1$$q151-178$$tCell And Tissue Research
000208281 909C0 $$0252112$$pLOB$$xU10345
000208281 909CO $$ooai:infoscience.tind.io:208281$$pSTI$$preview
000208281 917Z8 $$x206670
000208281 937__ $$aEPFL-REVIEW-208281
000208281 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000208281 980__ $$aREVIEW