Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics

Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm x 10 nm x 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 mu m thick) optimally sized and mounted for efficient, parallel FIB-SEM imaging. These chunks are imaged separately and then 'volume stitched' back together, producing a final three-dimensional data set suitable for connectome tracing.


Published in:
Nature Methods, 12, 4, 319-U55
Year:
2015
Publisher:
London, Nature Publishing Group
ISSN:
1548-7091
Laboratories:




 Record created 2015-05-29, last modified 2018-03-17


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