000208096 001__ 208096
000208096 005__ 20190317000157.0
000208096 0247_ $$2doi$$a10.1038/srep09808
000208096 022__ $$a2045-2322
000208096 02470 $$2ISI$$a000354108700002
000208096 037__ $$aARTICLE
000208096 245__ $$aA versatile clearing agent for multi-modal brain imaging
000208096 269__ $$a2015
000208096 260__ $$bNature Publishing Group$$c2015$$aLondon
000208096 300__ $$a9
000208096 336__ $$aJournal Articles
000208096 520__ $$aExtensive mapping of neuronal connections in the central nervous system requires high-throughput mm-scale imaging of large volumes. In recent years, different approaches have been developed to overcome the limitations due to tissue light scattering. These methods are generally developed to improve the performance of a specific imaging modality, thus limiting comprehensive neuroanatomical exploration by multi-modal optical techniques. Here, we introduce a versatile brain clearing agent (2,2'-thiodiethanol; TDE) suitable for various applications and imaging techniques. TDE is cost-efficient, water-soluble and low-viscous and, more importantly, it preserves fluorescence, is compatible with immunostaining and does not cause deformations at sub-cellular level. We demonstrate the effectiveness of this method in different applications: in fixed samples by imaging a whole mouse hippocampus with serial two-photon tomography; in combination with CLARITY by reconstructing an entire mouse brain with light sheet microscopy and in translational research by imaging immunostained human dysplastic brain tissue.
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aCostantini, Irene
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aGhobril, Jean-Pierre
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aDi Giovanna, Antonino Paolo
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aMascaro, Anna Letizia Allegra
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aSilvestri, Ludovico
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aMuellenbroich, Marie Caroline
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aOnofri, Leonardo
000208096 700__ $$uUniv Florence, A Meyer Childrens Hosp, Dept Neurosci Pharmacol & Child Hlth, Pediat Neurol & Neurogenet Unit, I-50139 Florence, Italy$$aConti, Valerio
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aVanzi, Francesco
000208096 700__ $$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy$$aSacconi, Leonardo
000208096 700__ $$uUniv Florence, A Meyer Childrens Hosp, Dept Neurosci Pharmacol & Child Hlth, Pediat Neurol & Neurogenet Unit, I-50139 Florence, Italy$$aGuerrini, Renzo
000208096 700__ $$0240392$$g150822$$uEcole Polytech Fed Lausanne, Brain Mind Inst, Lab Neural Microcircuitry, CH-1015 Lausanne, Switzerland$$aMarkram, Henry
000208096 700__ $$uUniv Campus Biomed Rome, Dept Engn, I-00128 Rome, Italy$$aIannello, Giulio
000208096 700__ $$aPavone, Francesco Saverio$$uUniv Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy
000208096 773__ $$j5$$tScientific Reports$$k9808$$q1-9
000208096 8564_ $$uhttps://infoscience.epfl.ch/record/208096/files/srep09808.pdf$$zn/a$$s4666791$$yn/a
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000208096 937__ $$aEPFL-ARTICLE-208096
000208096 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000208096 980__ $$aARTICLE