000207959 001__ 207959
000207959 005__ 20180913063117.0
000207959 0247_ $$2doi$$a10.1364/OE.23.013333
000207959 022__ $$a1094-4087
000207959 02470 $$2ISI$$a000354706800081
000207959 037__ $$aARTICLE
000207959 245__ $$aAutomated multi-parameter measurement of cardiomyocytes dynamics with digital holographic microscopy
000207959 269__ $$a2015
000207959 260__ $$aWashington$$bOptical Society of America$$c2015
000207959 300__ $$a15
000207959 336__ $$aJournal Articles
000207959 520__ $$aCompounds tested during drug development may have adverse effects on the heart; therefore all new chemical entities have to undergo extensive preclinical assessment for cardiac liability. Conventional intensity-based imaging techniques are not robust enough to provide detailed information for cell structure and the captured images result in low-contrast, especially to cell with semi-transparent or transparent feature, which would affect the cell analysis. In this paper we show, for the first time, that digital holographic microscopy (DHM) integrated with information processing algorithms automatically provide dynamic quantitative phase profiles of beating cardiomyocytes. We experimentally demonstrate that relevant parameters of cardiomyocytes can be obtained by our automated algorithm based on DHM phase signal analysis and used to characterize the physiological state of resting cardiomyocytes. Our study opens the possibility of automated quantitative analysis of cardiomyocyte dynamics suitable for further drug safety testing and compounds selection as a new paradigm in drug toxicity screens
000207959 6531_ $$aThree-dimensional image processing Cell analysis Medical and biological imaging Digital holography
000207959 700__ $$0240010$$aRappaz, Benjamin$$g168594
000207959 700__ $$aMoon, Inkyu
000207959 700__ $$aYi, Faliu
000207959 700__ $$aJavidi, Bahram
000207959 700__ $$aMarquet, Pierre
000207959 700__ $$0240363$$aTurcatti, Gerardo$$g103994
000207959 773__ $$j23$$k10$$q13333-13347$$tOptics Express
000207959 8564_ $$s653594$$uhttps://infoscience.epfl.ch/record/207959/files/CardioCellAnalysis%20Rev%20Moon%20BJ-BR%20finalREV.pdf$$yPreprint$$zPreprint
000207959 909C0 $$0252118$$pPTCB$$xU11378
000207959 909CO $$ooai:infoscience.tind.io:207959$$pSV$$particle
000207959 917Z8 $$x103994
000207959 917Z8 $$x182396
000207959 937__ $$aEPFL-ARTICLE-207959
000207959 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000207959 980__ $$aARTICLE