000207649 001__ 207649
000207649 005__ 20180317094440.0
000207649 0247_ $$2doi$$a10.1038/ncomms1961
000207649 022__ $$a2041-1723
000207649 037__ $$aARTICLE
000207649 245__ $$aHigh-performance and site-directed in utero electroporation by a triple-electrode probe
000207649 269__ $$a2012
000207649 260__ $$c2012
000207649 336__ $$aJournal Articles
000207649 520__ $$aIn utero electroporation is a powerful tool to transfect and manipulate neural-precursor cells of the rodent parietal cortex and their progeny in vivo. Although this technique can potentially target numerous brain areas, reliability of transfection in some brain regions is low or physical access is limited. Here we present a new in utero electroporation configuration based on the use of three electrodes, the relative position and polarities of which can be adjusted. The technique allows easy access and exceedingly reliable monolateral or bilateral transfection at brain locations that could only be sporadically targeted before. By improvement in the efficiency of the electrical field distribution, demonstrated here by a mathematical simulation, the multi-electrode configuration also extends the developmental timeframe for reliable in utero electroporation, allowing for the first time specific transfection of Purkinje cells in the rat cerebellum.
000207649 700__ $$aDal Maschio, Marco
000207649 700__ $$0249241$$aGhezzi, Diego$$g254787
000207649 700__ $$aBony, Guillaume
000207649 700__ $$aAlabastri, Alessandro
000207649 700__ $$aDeidda, Gabriele
000207649 700__ $$aBrondi, Marco
000207649 700__ $$aSato, Sebastian Sulis
000207649 700__ $$aZaccaria, Remo Proietti
000207649 700__ $$aDi Fabrizio, Enzo
000207649 700__ $$aRatto, Gian Michele
000207649 700__ $$aCancedda, Laura
000207649 773__ $$j3$$q960$$tNature Communications
000207649 8564_ $$s2737604$$uhttps://infoscience.epfl.ch/record/207649/files/Paper.pdf$$yPublisher's version$$zPublisher's version
000207649 909CO $$ooai:infoscience.tind.io:207649$$particle$$pSTI
000207649 909C0 $$0252540$$pLNE$$xU13047
000207649 917Z8 $$x254787
000207649 917Z8 $$x254787
000207649 937__ $$aEPFL-ARTICLE-207649
000207649 973__ $$aOTHER$$rNON-REVIEWED$$sPUBLISHED
000207649 980__ $$aARTICLE