A random-walk based model to explain ultrasensitive magnetic bead-based immunoassays

We introduce a new modeling approach for understanding the fundamental principle of bead-based surface coverage immunoassays that rely on the specific interaction between surface-bound “small” magnetic beads (1 μm in diameter) and a flow of “large” magnetic beads (3 μm in diameter). The latter ones carry target antigens and are simultaneously used as detection labels. This immunoassay technique allows attaining extremely low (attomolar) limits of detection, with strongly sub-linear dose-response curves, but the origin of these features remained unclear up to now. Our comprehensive theoretical model allows complete understanding of these unsolved issues.


Published in:
Proceed. MicroTAS 2014, 227-229
Presented at:
The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, TX, USA, 26-30 October
Year:
2014
Keywords:
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 Record created 2015-04-20, last modified 2018-03-17

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