000207378 001__ 207378
000207378 005__ 20190317000142.0
000207378 022__ $$a1615-9853
000207378 02470 $$2ISI$$a000350002600013
000207378 02470 $$a25407602$$2PMID
000207378 0247_ $$2doi$$a10.1002/pmic.201400355
000207378 037__ $$aARTICLE
000207378 245__ $$aA mammalian transcription factor-specific peptide repository for targeted proteomics
000207378 269__ $$a2015
000207378 260__ $$bWiley-Blackwell$$c2015$$aHoboken
000207378 300__ $$a5
000207378 336__ $$aJournal Articles
000207378 520__ $$aSite-specific transcription factors (TFs) play an essential role in mammalian development and function as they are vital for the majority of cellular processes. Despite their biological importance, TF proteomic data is scarce in the literature, likely due to difficulties in detecting peptides as the abundance of TFs in cells tends to be low. In recent years, significant improvements in MS-based technologies in terms of sensitivity and specificity have increased the interest in developing quantitative methodologies specifically targeting relatively lowly abundant proteins such as TFs in mammalian models. Such efforts would be greatly aided by the availability of TF peptide-specific information as such data would not only enable improvements in speed and accuracy of protein identifications, but also ameliorate cross-comparisons of quantitative proteomics data and allow for a more efficient development of targeted proteomics assays. However, to date, no comprehensive TF proteotypic peptide database has been developed. To address this evident lack of TF peptide data in public repositories, we are generating a comprehensive, experimentally derived TF proteotypic peptide spectral library dataset based on in vitro protein expression. Our library currently contains peptide information for 89 TFs and this number is set to increase in the near future. All MS data have been deposited in the ProteomeXchange with identifier PXD001212 (http://proteomecentral.proteomexchange.org/dataset/PXD001212).
000207378 6531_ $$aAnimal proteomics
000207378 6531_ $$aGene expression
000207378 6531_ $$aPeptide database
000207378 6531_ $$aSRM
000207378 6531_ $$aTargeted proteomics
000207378 6531_ $$aTranscription factors
000207378 700__ $$0244790$$g103733$$uUniv Basel, Biozentrum, CH-4056 Basel, Switzerland$$aSimicevic, Jovan
000207378 700__ $$aMoniatte, Marc
000207378 700__ $$aHamelin, Romain
000207378 700__ $$uUniv Basel, Biozentrum, CH-4056 Basel, Switzerland$$aAhrne, Erik
000207378 700__ $$uEcole Polytech Fed Lausanne, Lab Syst Biol & Genet, Inst Bioengn, Sch Life Sci, CH-1015 Lausanne, Switzerland$$aDeplancke, Bart$$g182376$$0244717
000207378 773__ $$j15$$tProteomics$$k4$$q752-756
000207378 909C0 $$xU11829$$0252247$$pUPDEPLA
000207378 909CO $$qGLOBAL_SET$$pSV$$ooai:infoscience.tind.io:207378$$particle
000207378 917Z8 $$x182376
000207378 937__ $$aEPFL-ARTICLE-207378
000207378 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000207378 980__ $$aARTICLE