000207342 001__ 207342
000207342 005__ 20190617200725.0
000207342 0247_ $$2doi$$a10.1242/jcs.160150
000207342 022__ $$a0021-9533
000207342 02470 $$2ISI$$a000349786500013
000207342 037__ $$aARTICLE
000207342 245__ $$aAnalysis of S. pombe SIN protein association to the SPB reveals two genetically separable states of the SIN
000207342 260__ $$bCompany Of Biologists Ltd$$c2015$$aCambridge
000207342 269__ $$a2015
000207342 300__ $$a14
000207342 336__ $$aJournal Articles
000207342 520__ $$aThe Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis, and asymmetric association of SIN proteins with the mitotic spindle pole bodies (SPBs) is important for its regulation. Here, we have used semi-automated image analysis to study SIN proteins in large numbers of wild-type and mutant cells. Our principal conclusions are: first, that the association of Cdc7p with the SPBs in early mitosis is frequently asymmetric, with a bias in favour of the new SPB; second, that the early association of Cdc7p-GFP to the SPB depends on Plo1p but not Spg1p, and is unaffected by mutations that influence its asymmetry in anaphase; third, that Cdc7p asymmetry in anaphase B is delayed by Pom1p and by activation of the spindle assembly checkpoint, and is promoted by Rad24p; and fourth, that the length of the spindle, expressed as a fraction of the length of the cell, at which Cdc7p becomes asymmetric is similar in cells dividing at different sizes. These data reveal that multiple regulatory mechanisms control the SIN in mitosis and lead us to propose a two-state model to describe the SIN.
000207342 6531_ $$aS. pombe
000207342 6531_ $$aCytokinesis
000207342 6531_ $$aSIN
000207342 700__ $$uEcole Polytech Fed Lausanne, Cell Cycle Control Lab, SV ISREC, CH-1015 Lausanne, Switzerland$$aWachowicz, Paulina
000207342 700__ $$uSwiss Inst Bioinformat, Swiss Prot Grp, CH-1015 Lausanne, Switzerland$$aChasapi, Anastasia
000207342 700__ $$0243132$$g181959$$uEcole Polytech Fed Lausanne, Cell Cycle Control Lab, SV ISREC, CH-1015 Lausanne, Switzerland$$aKrapp, Andrea
000207342 700__ $$aDel Rosario, Elena Cano
000207342 700__ $$uEcole Polytech Fed Lausanne, Biomed Imaging Grp, CH-1015 Lausanne, Switzerland$$aSchmitter, Daniel
000207342 700__ $$0240181$$g115533$$uEcole Polytech Fed Lausanne, Biomed Imaging Grp, CH-1015 Lausanne, Switzerland$$aSage, Daniel
000207342 700__ $$0240182$$g115227$$uEcole Polytech Fed Lausanne, Biomed Imaging Grp, CH-1015 Lausanne, Switzerland$$aUnser, Michael
000207342 700__ $$uSwiss Inst Bioinformat, Swiss Prot Grp, CH-1015 Lausanne, Switzerland$$aXenarios, Ioannis
000207342 700__ $$g182519$$aRougemont, Jacques$$0249227
000207342 700__ $$aSimanis, Viesturs$$uEcole Polytech Fed Lausanne, Cell Cycle Control Lab, SV ISREC, CH-1015 Lausanne, Switzerland$$g174228$$0240501
000207342 773__ $$j128$$tJournal Of Cell Science$$k4$$q741-754
000207342 8564_ $$uhttp://bigwww.epfl.ch/publications/wachowicz1501.html$$zURL
000207342 8564_ $$uhttp://bigwww.epfl.ch/publications/wachowicz1501.pdf$$zURL
000207342 8564_ $$uhttp://bigwww.epfl.ch/publications/wachowicz1501.ps$$zURL
000207342 909C0 $$xU10347$$0252054$$pLIB
000207342 909CO $$ooai:infoscience.tind.io:207342$$qGLOBAL_SET$$pSTI$$particle
000207342 917Z8 $$x115226
000207342 937__ $$aEPFL-ARTICLE-207342
000207342 970__ $$awachowicz1501/LIB
000207342 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000207342 980__ $$aARTICLE