000207017 001__ 207017
000207017 005__ 20190604054637.0
000207017 0247_ $$2doi$$a10.1021/acs.analchem.5b00455
000207017 022__ $$a1520-6882
000207017 02470 $$2ISI$$a000353429200061
000207017 037__ $$aARTICLE
000207017 245__ $$aElectrochemical push-pull probe: from scanning electrochemical microscopy (SECM) to multimodal altering of cell microenvironment
000207017 260__ $$bAmer Chemical Soc$$c2015$$aWashington
000207017 269__ $$a2015
000207017 300__ $$a8
000207017 336__ $$aJournal Articles
000207017 520__ $$aTo understand biological processes at the cellular level, a general approach is to alter the cells’ environment and to study their chemical responses. Herein, we present the implementation of an electrochemical push-pull probe, which combines a microfluidic system with a microelectrode, as a tool for locally altering the microenvironment of few adherent living cells by working in two different perturbation modes, namely electrochemical (i.e. electrochemical generation of a chemical effector compound) and microfluidic (i.e. infusion of a chemical effector compound from the pushing microchannel, while aspirating it through the pulling channel thereby focusing the flow between the channels). The effect of several parameters such as flow rate, working distance and probe inclination angle on the affected area of adherently growing cells was investigated both theoretically and experimentally. As a proof of concept, localized fluorescent labeling and pH changes were purposely introduced to validate the probe as a tool for studying adherent cancer cells through the control over the chemical composition of the extracellular space with high spatiotemporal resolution. A very good agreement between experimental and simulated results showed for instance, that the electrochemical perturbation mode enables to affect precisely only few living cells localized in a high-density cell culture.
000207017 700__ $$0245816$$g217921$$aBondarenko, Alexandra
000207017 700__ $$0242741$$g176551$$aCortes-Salazar, Fernando
000207017 700__ $$aGheorghiu, Mihaela
000207017 700__ $$aGaspar, Szilveszter
000207017 700__ $$0242750$$g193405$$aMomotenko, Dmitry
000207017 700__ $$aStanica, Luciana
000207017 700__ $$g208650$$aLesch, Andreas$$0246751
000207017 700__ $$aGheorghiu, Eugen
000207017 700__ $$aGirault, Hubert H.$$g105258$$0242739
000207017 773__ $$j87$$tAnalytical Chemistry$$q4479-4486
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000207017 8564_ $$uhttps://infoscience.epfl.ch/record/207017/files/ac5b00455_si_001.pdf$$zSupporting Information$$s6439634$$ySupporting Information
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000207017 8564_ $$uhttps://infoscience.epfl.ch/record/207017/files/acs2Eanalchem2E5b00455.pdf$$zPublisher's version$$s3293664$$yPublisher's version
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000207017 937__ $$aEPFL-ARTICLE-207017
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000207017 980__ $$aARTICLE