Coherent manipulation of free amino acids fluorescence

Coherent manipulation of molecular wavepackets in biomolecules might contribute to the quest towards label-free cellular imaging and protein identification. We report the use of optimally tailored UV laser pulses in pump-probe depletion experiments that selectively enhance or decrease fluorescence between two aromatic amino acids: tryptophan (Trp) and tyrosine (Tyr). Selective fluorescence modulation is achieved with a contrast of ∼35%. A neat modification of the time-dependent fluorescence depletion signal of Trp is observed, while the Tyr transient trace remains unchanged. The mechanism invoked for explaining the change of the depletion of Trp is a less efficient coupling between the fluorescing state and the higher non-radiative excited states by the optimally shaped pulse, than by the reference pulse. © 2012 the Owner Societies.


Published in:
Physical Chemistry Chemical Physics, 14, 26, 9317
Year:
2012
ISSN:
1463-9084
Laboratories:




 Record created 2015-03-12, last modified 2018-09-13


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