000206224 001__ 206224
000206224 005__ 20181203023814.0
000206224 0247_ $$2doi$$a10.1101/gad.254532.114
000206224 022__ $$a1549-5477
000206224 02470 $$2PMID$$a25737281
000206224 02470 $$a000351191100006$$2ISI
000206224 037__ $$aARTICLE
000206224 245__ $$aA KAP1 phosphorylation switch controls MyoD function during skeletal muscle differentiation
000206224 260__ $$bCold Spring Harbor Lab Press, Publications Dept$$c2015$$aCold Spring Harbor
000206224 269__ $$a2015
000206224 300__ $$a13
000206224 336__ $$aJournal Articles
000206224 520__ $$aThe transcriptional activator MyoD serves as a master controller of myogenesis. Often in partnership with Mef2 (myocyte enhancer factor 2), MyoD binds to the promoters of hundreds of muscle genes in proliferating myoblasts yet activates these targets only upon receiving cues that launch differentiation. What regulates this off/on switch of MyoD function has been incompletely understood, although it is known to reflect the action of chromatin modifiers. Here, we identify KAP1 (KRAB [Krüppel-like associated box]-associated protein 1)/TRIM28 (tripartite motif protein 28) as a key regulator of MyoD function. In myoblasts, KAP1 is present with MyoD and Mef2 at many muscle genes, where it acts as a scaffold to recruit not only coactivators such as p300 and LSD1 but also corepressors such as G9a and HDAC1 (histone deacetylase 1), with promoter silencing as the net outcome. Upon differentiation, MSK1-mediated phosphorylation of KAP1 releases the corepressors from the scaffold, unleashing transcriptional activation by MyoD/Mef2 and their positive cofactors. Thus, our results reveal KAP1 as a previously unappreciated interpreter of cell signaling, which modulates the ability of MyoD to drive myogenesis.
000206224 6531_ $$aKAP1
000206224 6531_ $$amyogenesis
000206224 6531_ $$aepigenetics
000206224 6531_ $$aMyoD
000206224 6531_ $$aMSK1 phosphorylation
000206224 6531_ $$aG9a
000206224 700__ $$aSingh, Kulwant
000206224 700__ $$0245611$$g214793$$aCassano, Marco
000206224 700__ $$aPlanet, Evarist
000206224 700__ $$aSebastian, Soji
000206224 700__ $$0247285$$g234439$$aJang, Suk Min
000206224 700__ $$aSohi, Gurjeev
000206224 700__ $$aFaralli, Hervé
000206224 700__ $$aChoi, Jinmi
000206224 700__ $$aYoun, Hong-Duk
000206224 700__ $$aDilworth, F Jeffrey
000206224 700__ $$aTrono, Didier$$g167919$$0240083
000206224 773__ $$j29$$tGenes & development$$k5$$q513-25
000206224 909C0 $$xU11172$$0252036$$pLVG
000206224 909CO $$pSV$$particle$$ooai:infoscience.tind.io:206224
000206224 917Z8 $$x169414
000206224 937__ $$aEPFL-ARTICLE-206224
000206224 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000206224 980__ $$aARTICLE